HMGB1 Protein (AA 1-215)

Catalog No. ABIN934963

Recombinant HMGB1 protein expressed in Baculovirus.

Quick Overview for HMGB1 Protein (AA 1-215) (ABIN934963)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus

Application: SDS-PAGE (SDS)

Purity: > 90 % pure

Product Details

Protein Characteristics: AA 1-215

Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH

Characteristics: Purified recombinant Human HMGB1 protein
Expression System: Baculovirus
Molecular weight on SDS-PAGE will appear higher.

Endotoxin Level: < 1.0 EU per µg of protein (determined by LAL method)

Application Details

Application Notes: HMGB1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris pH 8.0, containing 0 mM EDTA, 0.0 mM DTT, and 10 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background: High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Recombinant human HMGB1 was expressed in High Five insect cells and purified by conventional chromatography techniques.
Alternative Names: High-mobility group box1 protein, Sulfoglucuronyl carbohydrate binding protein protein, HMGB1 protein, HMGB 1 protein, Amphoterin protein, HMGB-1, HMG1 protein, High-mobility group box1 protein, HMG3 protein, HMGB 1, HMGB-1 protein, SBP-1 protein, HMGB1, high-mobility group (nonhistone chromosomal) protein 1 protein, DKFZp686A04236, high mobility group protein 1 protein

Molecular Weight: 25 kDa (223 AA)

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome