TCEB2 Protein (AA 1-118)

Catalog No. ABIN935006

This Recombinant TCEB2 protein is produced in Escherichia coli (E. coli).

Quick Overview for TCEB2 Protein (AA 1-118) (ABIN935006)

Target: TCEB2 (Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Protein Characteristics: AA 1-118

Sequence: MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCI EPFSSPPELP DVMKPQDSGS SANEQAVQ

Characteristics: Purified recombinant Human Elongin B protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher.

Application Details

Application Notes: Elongin B protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT, 0.0 mM PMSF, and 20 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: TCEB2 (Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2))

Alternative Name: Elongin B

Background: Elongin B , also known as TCEB2, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimer consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C). It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant TCEB2 was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: Elongin B protein, Transcription elongation factor B polypeptide 2 protein, Elongin 18 kDa subunit protein, SIII p18 protein, TCEB 2 protein, EloB protein, EloB protein, RNA polymerase II transcription factor SIII subunit B protein, Transcription elongation factor B (SIII) polypeptide 2., TCEB2 protein

Molecular Weight: 13.1 kDa (118 AA)

Pathways: SARS-CoV-2 Protein Interactome