IFNB1 Protein
Quick Overview for IFNB1 Protein (ABIN935242)
Target
See all IFNB1 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Sequence
- MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN
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Characteristics
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Purified recombinant Human IFN beta protein
Expression System: CHO cells
Bioactivity: Assay#1: The biological activity of PeproTech's human IFN-gamma was based on analysis using the WISH cell line with VSV as the challenge virus. The specific activitywas determined to be >5 x 10 8 units/mg, Assay#2: Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/mL. -
Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
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Interferon, beta 1, Fibroblast (IFNB1) (AA 22-182) protein (His tag)
IFNB1 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Interferon, beta 1, Fibroblast (IFNB1) (AA 22-187) protein (Fc Tag)IFNB1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interferon, beta 1, Fibroblast (IFNB1) (AA 22-182) protein (His tag)IFNB1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95% by SDS-PAGE SDS
Interferon, beta 1, Fibroblast (IFNB1) (AA 1-187) protein (GST tag)IFNB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interferon, beta 1, Fibroblast (IFNB1) (AA 22-187), (full length) protein (GST tag)IFNB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in water to a concentration of 0.1-1.0 g/mL. Do not vortex. For extended storage, dilute further in a buffer containing a carrier protein (ie: 0.1 % BSA).
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Buffer
- Lyophilized from 10 mM sodium citrate, with 100 mM NaCl, and 10 mM Sucrose.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))
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Alternative Name
- IFN beta
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Background
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Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFNa is an acid stable molecule that signals through IFNa/betaR, which is also used by IFNbeta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFNa contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.
Alternative Names: Leukocyte interferon antibody, RMMB1 antibody, Interferon beta antibody, Type I interferon antibody, B cell interferon antibody -
Molecular Weight
- 20 kDa
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Pathways
- JAK-STAT Signaling, TCR Signaling, TLR Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome
Target
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