IL-15 Protein
Quick Overview for IL-15 Protein (ABIN935477)
Target
See all IL-15 (IL15) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Characteristics
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Purified recombinant Human IL15 protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 was found to be
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Interleukin 15 (IL15) (AA 49-162) protein
IL15 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by Tris-Bis PAGE, > 95 % as determined by SEC-HPLC
Interleukin 15 (IL15) (AA 50-162) (Active) protein (His tag)IL15 Origin: Human Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Interleukin 15 (IL15) (AA 49-162) protein (His-IF2DI Tag)IL15 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 15 (IL15) (AA 1-162) protein (GST tag)IL15 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 15 (IL15) (AA 49-162), (full length) protein (His tag)IL15 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag)IL15 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (His tag)IL15 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) proteinIL15 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Comment
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Biological activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 was found to be
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Assay Procedure
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Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in sterile water at the stock concentration of at elast 0.1 mg/mL.
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Buffer
- Lyophilized from 20 mM Tris, pH 8.0.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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- IL-15 (IL15) (Interleukin 15 (IL15))
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Alternative Name
- IL15
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Background
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Interleukin 15 (IL-15) is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through the IL-2/IL-15 beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces cell proliferation of natural killer cells, cells of the innate immune system whose principal role is to kill virally infected cells.
Alternative Names: IL-15 protein, IL-15 protein, IL 15 protein, IL-15, IL 15, IL15, Interleukin 15 protein, MGC9721 protein, IL-15 protein, IL-T protein -
Pathways
- JAK-STAT Signaling, Glycosaminoglycan Metabolic Process
Target
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