IGF1 Protein
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- Target See all IGF1 Proteins
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Characteristics
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Purified recombinant Human IGF1 protein
Expression System: E.coli
Bioactivity: The ED50, calculated by the dose-dependant proliferation of murine BALB\\C 3T3 cells (measured by 3H-thymidine uptake) is - Purity
- > 98 % pure
- Top Product
- Discover our top product IGF1 Protein
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Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) (Active) protein
IGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) proteinIGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Insulin-Like Growth Factor 1 (IGF1) protein (Fc Tag)IGF1 Origin: Mouse Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Insulin-Like Growth Factor 1 (IGF1) (AA 1-115) (Active) proteinIGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS Active
Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) protein (His tag)IGF1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) protein (His-IF2DI Tag)IGF1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Insulin-Like Growth Factor 1 (IGF1) (AA 49-138) protein (GST tag)IGF1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Assay Procedure
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Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in distiled water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- Supplied as a lyophilized powder.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
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- Target
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
- Alternative Name
- IGF1 (IGF1 Products)
- Background
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The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH, MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2, MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986, Rosenfeld, 2003).
Alternative Names: IGF-IA protein, IGF1 protein, Somatomedin C protein, MGF protein, IGF-1 protein, IGF-1, IGF-I protein, Mechano growth factor protein, IGF 1, Insulin-Like Growth Factor 1 protein, IGFI protein, IGF1, IGF 1 protein - Molecular Weight
- 7.655 kDa
- Pathways
- RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
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