KIT Ligand Protein (KITLG) (full length)

Catalog No. ABIN935785

Product Details

Target: KIT Ligand (KITLG)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: full length

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: Purified recombinant Human SCF protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is

Purification: Proprietary chromatographic technique

Purity: > 98 % pure

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Comment:

Biological activity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is

Assay Procedure:

Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water not less than 100 μg/mL.

Buffer: Lyophilized from a concentrated (1 mg/mL) solution in water containing 11 mM CH3COOH.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details

Target: KIT Ligand (KITLG)

Alternative Name: SCF (KITLG Products)

Synonyms: Xkl-1 Protein, Xsl Protein, Xsl-1 Protein, Xsl-2 Protein, steel Protein, KITLG Protein, SCF Protein, kitl Protein, kl-1 Protein, mgf Protein, scf Protein, FPH2 Protein, KL-1 Protein, Kitl Protein, MGF Protein, SF Protein, SHEP7 Protein, Clo Protein, Con Protein, Gb Protein, Kitlg Protein, Mgf Protein, SLF Protein, Sl Protein, blz Protein, contrasted Protein, 710-712 Protein, CSF Protein, KITL Protein, KIT ligand L homeolog Protein, KIT ligand Protein, kit ligand Protein, kitlg.L Protein, KITLG Protein, kitlg Protein, Kitl Protein, Kitlg Protein

Background: Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as steel factor or c-kit ligand. SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
Alternative Names: SCF protein, MGF protein, Mast cell growth factor protein, Kitl protein, c-Kit Ligand protein, Steel Factor protein, KL-1 protein, MGF protein, C-kit ligand protein, SF protein, Mast Cell Growth Factor protein, Kit ligand Precursor protein, DKFZp686F2250, Stem Cell Factor protein

Molecular Weight: 18.409 Da

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway