CD14 Protein (CD14) (Soluble)
Quick Overview for CD14 Protein (CD14) (Soluble) (ABIN935793)
Target
See all CD14 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- Soluble
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Sequence
- TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P
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Characteristics
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Purified recombinant Human sCD14 protein
Expression System: 293 cells
Bioactivity: Determined by the dose dependent activation of NF-kappaB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/ul to 200 ng/ul. The NF-kappaB activation is enhanced when the assay is done in the presence of 0.25 ng/ul to 1.0 ng/ul bacterial LPS. -
Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
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CD14 (CD14) (AA 16-345) protein (His tag)
CD14 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 20-344) protein (His tag)CD14 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 1-375) protein (GST tag)CD14 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD14 (CD14) (AA 20-352) protein (His tag)CD14 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
CD14 (CD14) (AA 20-349) protein (His tag)CD14 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
CD14 (CD14) protein (His tag,Fc Tag)CD14 Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
CD14 (CD14) protein (His tag)CD14 Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
CD14 (CD14) protein (Fc Tag)CD14 Origin: Rat Host: HEK-293 Cells Recombinant > 93 % as determined by SDS-PAGE
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Supplied as a lyophilized powder.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- CD14
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Alternative Name
- sCD14
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Background
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CD14 is a cell surface anchored glycoprotein that is expressed predominantly by monocytes and tissue macrophages. CD14 associates with MD-2 (LY-96) and TLR4 to form a receptor complex, which signals specifically in response to bacterial lipopolysaccharide (LPS) binding. The CD14/MD-2/TLR4 receptor complex signals via MyD88, TIRAP, and TRAF6, and ultimately activates NF-kappa-beta. CD14 also exists in a soluble form, designated as sCD14, which is capable of specifically binding LPS in the extracellular space.
Alternative Names: sCD-14 protein, sCD-14, sCD-14 protein, sCD 14 protein, sCD14, soluble CD14 protein, sCD 14 -
Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Target
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