TNF alpha Protein

Catalog No. ABIN935843

This Recombinant TNF alpha protein is produced in Escherichia coli (E. coli). There is 1 publication available.

Quick Overview for TNF alpha Protein (ABIN935843)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % pure

Product Details

Sequence: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Characteristics: Purified recombinant Human TNF alpha protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the cytolysis of Mouse L929 cells in the presence of Actinomycin D is ? 0.05 ng/mL, corresponding to a specific activity of ? 2 x 10^7 units/mg.

Endotoxin Level: < 0.1 ng per μg (1 EU/μg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Comment:

Biological activity: The ED50 as determined by the cytolysis of Mouse L929 cells in the presence of Actinomycin D is approx. 0.05 ng/mL, corresponding to a specific activity of approx. 2 x 10^7 units/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration of 0.1 - 1.0 mg/mL. Allow to sit at room temperature for 2 hours before use.

Buffer: Lyophilized from 3 mM Tris, pH 8.0.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

References

Spatz, Eibl, Hink, Wolf, Fischer, Mayr, Schernthaner, Eibl: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." in: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, (2003) (PubMed).

Target Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.
Alternative Names: TNFSF2 protein, Necrosin protein, TNF-a protein, Cachectin protein, Cytotoxin protein, TNFa protein, DIF protein, Tumor Necrosis Factor protein, TNF-alpha protein, Differentiation-inducing factor protein

Molecular Weight: 17.4 kDa

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome