Protein A Protein (Alkaline Phosphatase (AP))
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- Target See all Protein A Proteins
- Protein A
- Protein Type
- Native
- Origin
- Staphylococcus aureus
- Host
- Please inquire
- Purification tag / Conjugate
- This Protein A protein is labelled with Alkaline Phosphatase (AP).
- Application
- ELISA
- Specificity
- Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A. Protein A Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein A. No reaction was observed against anti-Protein G.
- Top Product
- Discover our top product Protein A Protein
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Protein A protein (HRP)
Origin: Staphylococcus aureus ELISA, WB
Protein A protein (HRP)Origin: Staphylococcus aureus Native ELISA, WB
Protein A protein (Sepharose)Origin: Staphylococcus aureus Native IP
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- Application Notes
- Protein A Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
- Comment
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Protein A Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target
- Protein A
- Abstract
- Protein A Products
- Synonyms
- alpha protein Protein, repB Protein
- Background
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Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.
Synonyms: ProA, ALP, Alk Phos
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