GM-CSF Protein

Catalog No. ABIN987887

This GM-CSF protein is produced in Escherichia coli (E. coli).

Quick Overview for GM-CSF Protein (ABIN987887)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Biological Activity: Active

Origin: Rhesus Monkey

Source: Escherichia coli (E. coli)

Purity: > 98 % by SDS-PAGE and HPLC analyses.

Product Details

Sequence: APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTV EVVSEMFDLQ EPSCLQTRLE LYKQGLQGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFQSFKENL KDFLLVIPFD CWEPVQ

Characteristics: Fully biologically active when compared to standard. The ED50 as calculated by the dose-dependant stimulation of the proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to aof 1.0×107 IU/mg

Endotoxin Level: Level Less than 1EU/µg of rRhGM-CSF as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at

Storage: 4 °C

Target Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)

Background: GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. Synonym: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Rhesus Macaque. Formulation: Lyophilized from a 0.2µm filtered concentratedcsolution in PBS, pH 7.4.

Molecular Weight: Approximately 14.4 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin