GM-CSF Protein

Catalog No. ABIN987972

Recombinant GM-CSF protein expressed in Escherichia coli (E. coli).

Quick Overview for GM-CSF Protein (ABIN987972)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE and Commassie blue staining

Product Details

Sequence: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQana lysis The sequence of the first five N-terminal amino acids has been found to be (Met)-Ala-Pro-Ala-Arg-Ser

Characteristics: Determined by the dose-dependant stimulation of human TF-1 cells. The ED50 for this effect is 0.24-0.36 ng/ml.

Endotoxin Level: Level Less than 0.1 ng/µg (1 EU/myg) determined by LAL tes

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Target Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: Granulocyte Macrophage Colony Stimulating Factor

Background: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. Synonym: GM-CSF, E.coli2. Formulation: Lyophilized from a 0.2 mym filtered solution in 2 mM PB, pH 7.4.

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin