IL-15 Protein

Catalog No. ABIN988042

This Recombinant IL-15 protein is produced in Escherichia coli (E. coli). There is 1 publication available.

Quick Overview for IL-15 Protein (ABIN988042)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98.0 % as determined by:(a)Analysis by RP-HPLC(b)Anion-exchange FPLC(c)Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Product Details

Sequence: MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISHESGDTDI HDTVENLIIL ANNILSSNGN ITESGCKECE ELEEKNIKEF LQSFVHIVQM FINTanalys is The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Trp-Val-Asn-Val. p to grams level.

Characteristics: The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be

Endotoxin Level: Level Less than 0.1 ng/µg (1 IEU/µg) of Recombinant Human Interleukin 15 (IL-15

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended to reconstitute the lyophilized Recombinant Human Interleukin 15 (IL-15) in sterile 18 M-omega-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard.

Storage: -20 °C

References

Stone, Chervin, Schreiber, Kranz: "Design and characterization of a protein superagonist of IL-15 fused with IL-15R? and a high-affinity T cell receptor." in: Biotechnology progress, Vol. 28, Issue 6, pp. 1588-97, (2012) (PubMed).

Target Details

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Alternative Name: Interleukin-15 (IL-15)

Background: Interleukin 15 (IL-15) is a cytokine that possesses a variety of biological functions. Interleukin 15 (IL-15) stimulates the proliferation of T-lymphocytes, which requires interaction of IL-15 with components of IL-2R, including IL-2R and probably IL-2R gamma, but not IL-2R. Recombinant Human Interleukin 15 (IL-15) produced in E. coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12,774 Da. Synonym: rHuIL-15, rHuIL15, Interleukin-15, Interleukin1. Formulation: The protein was lyophilized after extensive dialysis against 20mM PB, pH7.5, 150mM NaCl buffer.

Molecular Weight: 12,774 Da

Pathways: JAK-STAT Signaling, Glycosaminoglycan Metabolic Process