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VEGF Protein

VEGF Origin: Mouse Host: Yeast (Pichia pastoris) > 97 % by SDS-PAGE. Active
Catalog No. ABIN988283
  • Target See all VEGF Proteins
    VEGF (Vascular Endothelial Growth Factor (VEGF))
    Biological Activity
    Active
    Origin
    • 12
    • 6
    • 1
    • 1
    Mouse
    Source
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    Yeast (Pichia pastoris)
    Characteristics
    Resuspend cells in assay media (Earle Salts 199X Gibco #11150, without h-EGF) containing 10 % HI-FBS and transfer 100 ul/well to assay plate (5,100 cells/well passage#2). Serial dilute murine VEGF in assay media containing 10 % HI-FBS and transfer 100ml/well to cells in assay plate. Final assay volume is 200 ml/well, containing 10 % HI-FBS, and murine VEGF as indicated 89 hours. Add 20 ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well, Incubate 37°C and read at OD 490 nm. The ED50 for the effect is typically 4.1 - 6.2 ng/ml.
    Purity
    > 97 % by SDS-PAGE.
    Endotoxin Level
    level is less than 0.1 ng per myg (1 EU/myg) of VEGF
    Top Product
    Discover our top product VEGF Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Storage
    -20 °C
  • Target
    VEGF (Vascular Endothelial Growth Factor (VEGF))
    Alternative Name
    Vascular Endothelial Growth Factor (VEGF Products)
    Background
    VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors, fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Synonym: VEGF 6. Formulation: Lyophilized from 25 mM HEPES and 150 mM NaCl, pH 7.0.
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