VEGFA Protein

Catalog No. ABIN988286

This VEGFA protein is produced in Escherichia coli (E. coli).

Quick Overview for VEGFA Protein (ABIN988286)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 96 % by SDS-PAGE and HPLC analyses.

Product Details

Sequence: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR

Characteristics: Measured by its ability to induce proliferation of human umbilical vein endothelial cells. The ED50 for this effect is typically 2 - 4 ng/mL,corresponding to a specific activity of > 2.5×105 units/mg.

Endotoxin Level: Level Less than 1EU/µg of rmVEGF120 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: Vascular Endothelial Growth Factor 120 (VEGF120)

Background: VEGF was initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and by a variety of transformed cell lines as a mitogen specific for vascular endothelial cells. It was subsequently found to be identical to an independently discovered vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based on its ability to increase the permeability of capillary blood vessels. Three mouse cDNA clones, which arise through alternative splicing and which encode mature mouse monomeric VEGF having 120, 164, or 188, amino acids, respectively, have been identified. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 (the mouse homologue of human KDR), both members of the type III subclass of RTKs containing seven immunoglobulin-like repeats in their extracellular domains, have been shown to bind VEGF with high affinity. The roles of the homodimers of KDR, Flt, and the heterodimer ofKDR/Flt in VEGF signal transduction remain to be elucidated.In vivo, VEGF has been found to be a potent angiogenesis inducer. Synonym: Vascular Endothelial Growth Factor 120 (VEGF120), Mouse. Formulation: Lyophilized from a 0.2µm filtered solution in PBS, pH 7.4.

Molecular Weight: Recombinant mouse VEGF120 is a 28.4 kDa disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains.

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling