Choose an anti-IgG secondary antibody by matching the species in which your primary antibody was raised, then refine your choice by IgG specificity, cross-adsorption, antibody format, conjugate, and application. Use the quick selector below to reach a relevant product category, or continue to the IgG overview to learn how IgG structure affects secondary-antibody binding.
Product selection
Start with the host species of your primary antibody—not the species of the sample—then select the required specificity, conjugate, and application.
Find an anti-IgG secondary antibodyScientific information
Review IgG structure and the practical differences between H+L-, Fc-, Fab-, and subclass-specific detection.
Go to the IgG overviewFive checks before selection
Use these five checks before opening a product page. If critical information is missing, continue with the full selection guide rather than treating a product as automatically compatible.
A primary antibody raised in mouse requires an anti-mouse secondary antibody; a rabbit primary requires an anti-rabbit secondary. Do not confuse the primary-antibody host species with the species of the sample.
Choose H+L for broad recognition of heavy- and light-chain epitopes. Consider Fc-, heavy-chain-, Fab-, or subclass-specific detection when the experiment requires more selective recognition. Confirm the stated specificity on the individual product page.
Consider a cross-adsorbed secondary when immunoglobulins from the sample species or other antibodies in a multiplex experiment could cause unwanted binding. Check the exact adsorbed species or materials listed for the individual product.
Whole IgG is suitable for many general detection workflows. Fab or F(ab')2 fragments may be useful when Fc-mediated interactions, tissue penetration, or a specialized multiplex design affects the experiment.
Select HRP, alkaline phosphatase, biotin, or a fluorophore according to the detection system. Confirm whether the individual product lists the intended application as tested or only suggested, for example Western blot, ELISA, IHC, immunofluorescence, or flow cytometry.
Use the complete guide when the experiment includes species-on-species detection, same-host multiplex panels, fragments, direct labels, IP-Western, or incomplete metadata.
Secondary Antibody Selection GuideRockland secondary antibodies can be considered when the product matches your primary-antibody host species, required IgG specificity, cross-adsorption needs, format, conjugate, and intended application. Verify these attributes and current availability on the individual product page before selection.
Rabbit primary + HRP
Rockland 611-1302-1.0 · ABIN7539583
Manufacturer-tested: ELISAManufacturer-tested: WB
Mouse primary + HRP
Rockland 610-1302-1.0 · ABIN7539584
Manufacturer-tested: ELISAManufacturer-tested: WB
For Rabbit IP-Western workflows, Rabbit TrueBlot® may be considered when reduced and denatured immunoprecipitating IgG chains would interfere with target detection. Follow the current product protocol and confirm that the workflow conditions match your experiment.
An anti-IgG secondary antibody binds defined regions of an IgG primary antibody. The correct choice starts with the species in which the primary antibody was raised and then depends on the required IgG region, antibody format, sample context, application, and readout.
IgG is composed of two heavy and two light chains. This structure creates several possible recognition strategies for secondary antibodies. Broader recognition can be useful for signal generation, while more selective recognition may be required to distinguish subclasses, fragments, or assay components.
Recognizes heavy- and light-chain epitopes. Broad recognition does not provide subclass or Fc specificity.
Targets the Fc region. Confirm that the primary format contains an Fc region before selection.
May support experiments involving fragments or workflows where Fc-mediated interactions matter.
Can distinguish defined IgG subclasses when the product evidence and complete panel support that use.
Creative mind of antibodies-online with a keen eye for details. Proficient in the field of life-science with a passion for plant biotechnology and clinical study design. Responsible for illustrated and written content at antibodies-online as well as supervision of the antibodies-online scholarship program. Head of the Independent Validation Initiative, a program that helps researchers select reliable antibodies through independent performance testing.
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