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MeDIP, hMeDIP, bisulfite sequencing, and TAB-seq

The covalent addition of a methyl-group at the 5-carbon of cytosine in DNA, resulting in 5-methylcytosine (5-mC), is an epigenetic mark that influences gene expression, cell differentiation and embryonic development. DNA methyltransferase (DNMT) enzymes catalyze the process. The global methylation pattern in mammals is rather homogenous. However, the CpG islands, which are the main sites for methylation, tend to be near transcription start sites. Furthermore, the methylation level can differ significantly within one organism based on tissue type and between normal and cancerous cell in the same tissue.

While 5-mC is the most common and widely studied DNA-methylation cytosine methylation and demethylation processes give rise to various other forms. In particular 5-hydroxymethylcytosine (5-hmC) has garnered interest since it has been found to be elevated in certain mammalian tissues, such as human and mouse brain, as well as in embryonic stem cells. It is thought to determine developmental fates and, when present in aberrant patterns, contributes to disease. A complete understanding of the potential function 5-hmC requires an accurate, scalable sequencing method.

Assays to study DNA methylation

5mC and 5hmC assays

Traditionally, 5-mC sites are identified by bisulfite sequencing or methylated CpG island recovery assay (MIRA). These techniques however cannot distinguish between 5-mC and 5-hmC. TAB-seq (Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine) is a modification of the standard bisulfite sequencing method through which 5-hydroxymethylcytosine (5-hmC) can be specifically identified. In conjunction with the traditional methods TAB-seq allows mapping of both methylation variants at single-base resolution either on a genomic scale or for a particular locus. TAB-seq was first introduced in 2012. Until now it has been used to study the role of 5-hmC in brain functions, cancers and several cognitive disorders.

Methylated DNA immunoprecipitation (MeDIP) and hydroxylmethylated DNA immunoprecipitation (hMeDIP) on the other hand are two methods that take advantage of 5-mC- and 5-hmC-specific antibodies respectively to enrich DNA fragments bearing these modifications.

antibodies-online is proud to offer products to support your ventures into the DNA methylome with products for MeDIP, hMeDIP, bisulfite sequencing and TAB-seq. Please refer to the table below to find a specific product or get in touch with one of our customer service agents. If you are specifically looking for TAB-seq kits head over to our portal for genomics products genomics-online.

DNA methylation related products

Product Name Product Id Application Description Supplier
5-hmC antibody ABIN2486985 hMeDIP hMeDIP validated polyclonal 5-hmC antibody for a successful hMeDIP assay. Biovision
5-hmC antibody ABIN3199278 hMeDIP hMeDIP validated recombinant 5-hmC antibody for a successful hMeDIP assay. RevMAb Biosciences
5-mC antibody ABIN3071645 MeDIP MeDIP validated monoclonal 5-mC antibody for a successful MeDIP assay. Chromatrap
5-mC Antibody with Positive Primer Set ABIN3071710 MeDIP MeDIP validated monoclonal 5-mC antibody and positive primer set provide a complete set of tools for a successful MeDIP assay. Chromatrap
5-mC Antibody with Positive and Negative Primer Sets ABIN3071700 MeDIP MeDIP validated 5-mC antibody and control primer sets provide a complete set of tools for a successful MeDIP assay. Chromatrap
5-mC antibody ABIN2668983 MeDIP, MeDIP-Seq Highly characterized MeDIP and MeDIP-Seq validated monoclonal 5-mC antibody. Active Motif
5-mC antibody ABIN2668970 MeDIP MeDIP validated polyclonal 5-mC antibody for a successful MeDIP assay. Active Motif
5-hmC antibody ABIN2668979 hMeDIP Highly characterized hMeDIP validated monoclonal 5-hmC antibody. Active Motif
5-hmC antibody ABIN2668981 hMeDIP Highly characterized hMeDIP validated polyclonal 5-hmC antibody. Active Motif
MeDIP Kit ABIN2866109 MeDIP This MeDIP Kit is a fast, streamlined approach to immunoprecipitate and enrich for single-stranded DNA fragments containing 5-mC. Active Motif
hMeDIP Kit ABIN2866110 hMeDIP This hMeDIP Kit contains a high affinity 5-hmC antibody for efficient immunoprecipitation and enrichment of double or single stranded DNA fragments containing 5-hmC. Active Motif
Hydroxymethyl Collector ABIN2866111 hMeDIP A fast and simple process for enrichment of 5-hmC methylated genomic DNA fragments. Active Motif
HypoMethylCollector ABIN2866131 MeDIP, hMeDIP A novel technology for enrichment of unmethylated genomic DNA fragments for use in many downstream applications. Active Motif
MethylCollector Ultra ABIN2866132 MeDIP A novel technology capable of quickly and efficiently isolating methylated CpG islands from fragmented genomic DNA. Active Motif
β-Glucosyltransferase ABIN2669404 TAB-seq β-Glucosyltransferase can be used to glucosylate 5-hmC DNA. Active Motif
Tet Methylcytosine Dioxygenase 1 (TET1) ABIN2669628 TAB-seq Tet1 is a cytosine oxygenase that catalyzes the conversion of the modified genomic base 5-mC into 5-hydroxymethylcytosine (5-hmC). Active Motif
Methylated DNA Standard Kit ABIN2866136 MeDIP, hMeDIP DNA standards that can be used as positive controls in methylation analysis experiments. Active Motif
Bisulfite Conversion Kit ABIN2866119 Bisulfite sequencing, TAB-seq Bisulfite Conversion Kit that simplifies the analysis of DNA methylation by providing optimized conversion reagents and an easy-to-use protocol. Active Motif
GenoMatrix Whole Genome Amplification Kit ABIN2866101 Whole Genome Amplification Bias-free amplification of human genomic DNA. Active Motif


Stefan Pellenz, Ph.D.
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