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Dr. Stefan Pellenz, PhD

Product Manager at antibodies-online.com
Alumni of: Institut Pasteur

Goal-oriented, time line driven scientist, proficiently trained in different academic institutions in Germany, France and the USA. Experienced in the life sciences e-commerce environment with a focus on product development and customer relation management.

Articles by Stefan Pellenz

Loading Controls

Western blotting is a commonly used method to determine specific protein levels in a sample (e.g. tissue homogenate, extract). Loading control antibodies... Read more

Western Blotting (Immunoblot): Gel Electrophoresis for Proteins

Western Blotting (also called immunoblotting) is a technique used for analysis of individual proteins in a protein mixture (e.g. a cell lysate). In Western blotting (immunoblotting) the protein mixture is applied to a gel electrophoresis in a carrier matrix. Read more

What is Flow Cytometry (FACS Analysis)?

Originally developed in the late 1960s, flow cytometry is a popular analytical cell-biology technique that utilizes light to count and profile cells in a heterogenous fluid mixture. Flow cytometry is a particularly powerful. Read more

Monoclonal antibodies

In 1975 G. Köhler and C. Milstein published in Nature the introduction of a hybridoma technique to produce monoclonal antibodies. This technique has become a pillar in modern biological science Read more

An Introduction to ELISA (Part 1)

While there are many different kinds of ELISAs, the basic principle behind the assay is strikingly simple: antibodies conjugated to an enzyme (most commonly a peroxidase) are bound to an analyte that has been immobilized on a solid support (commonly a 96-well microtiter plate). Read more

An Introduction to ELISA (Part 2)

ELISA is an exceptionally powerful and versatile technique, and there are number of different ways to approach it. This article covers the various ELISA formats that are most commonly commercially available, and discusses the nuanced differences between them. Read more

The Epigenome Project

The frugality of the human genome contrasts with the profound complexity and diversity of the human species, and poses a number of interesting questions. Namely, how do you build such a stunningly complex system with so few distinguishable, functional units? Read more

Antibody and immunoglobulin alternatives (Part 1)

There is mounting pressure to develop and optimize alternatives to traditional immunoglobulins. Both products derived from immunoglobulins and non Ig-based alternatives are rising in popularity... Read more

Antibody and immunoglobulin alternatives (Part 2)

The common characteristic for antibody mimetics is the intention to provide alternatives that perform similar or better than antibodies. Their origins are very diverse and accordingly there is a wide... Read more

Protein Tumor Markers

Biomarkers that are produced at higher levels in cancerous conditions compared to benign conditions are collectively termed tumor markers. Most but not all of these substances are proteins that can be measured. Read more

Immunoprecipitation, CoIP, and ChIP-seq

At its core, immunoprecipitation is used to isolate a single protein. Various evolutions have turned this simple method into one of the mainstays in life science research. Read more

Protein Exosome Marker

Exosomes are small (50-120nm) endosome derived extracellular microvesicles (EMV). They can contain a vast array of different proteins depending on their host cell which, and their components are further modulated by cellular state (e.g. stress or activation, or inhibition of specific signaling pathways). Read more

MeDIP, hMeDIP, bisulfite sequencing, and TAB-seq

Modification at the 5-carbon of cytosine in DNA is an epigenetic mark that influences gene expression, cell differentiation and embryonic development. While 5-mC is the most common and widely studied DNA-methylation cytosine methylation and demethylation processes give rise to various other forms. Read more

Nanodiscs

Nanodiscs are soluble synthetic phospholipid bilayers that are encircled by a belt of helical membrane scaffold proteins. They enable reconstitution and detailed study of membrane proteins. Read more

Independent Validation Initiative

Most commercially available antibodies lack critical validation data. This is why we started the (IVI) to independently validate commercialantibodies and ELISA kits. The Independent Validation Initiative is centered around core values of transparency, reproducibility, scientific integrity. Read more

Share your validation data and get your money back

Be part of our Independent Validation Initiative. Validate one of our products and get your money back. Read more

antibodies-online SARS-CoV-2 Handbook

antibodies-online SARS-CoV-2 Handbook Read more

COVID-19 Research Products

COVID-19 Research, COVID-19 vaccine development, COVID-19 drug discovery, COVID-19 diagnostics development Immune response, HLA phenotyping, inflammatory response, cytokine release syndrome, cytokine storm, ARDS, blood clotting, autophagy Read more

SARS-CoV-2 Antibodies

antibodies-online has served hundreds of research institutions around the world with SARS-CoV-2 Antibodies. If you are looking for the right SARS-CoV-2 Antibody to suit your research setting, or kit development, we have grouped our current SARS-CoV-2 Anti Read more

SARS-CoV-2 Non-Structural Proteins

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SARS-CoV-2 Neutralizing Antibodies

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Mutations of SARS-CoV-2 S Protein

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SARS-CoV-2 Interferon Antagonism

Interferon Antagonism Read more

SARS-CoV-2 Cytokine Storm

Cytokine Release Syndrome – the “Cytokine Storm” Read more

SARS-CoV-2 Mutations Tracker

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SARS-CoV-2 Mutations Tracker

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SARS-CoV-2 Mutations Tracker

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Publications by Stefan Pellenz

Pellenz, Phelps, Tang, Hovde, Sinit, Fu, Li, Chen, Monnat: "New Human Chromosomal Sites with "Safe Harbor" Potential for Targeted Transgene Insertion." in: Human gene therapy, Vol. 30, Issue 7, pp. 814-828, (2020) (PubMed).

Pellenz, Monnat: "Identification and analysis of genomic homing endonuclease target sites." in: Methods in molecular biology (Clifton, N.J.), Vol. 1123, pp. 245-64, (2014) (PubMed).

Li, Pellenz, Ulge, Stoddard, Monnat: "Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins." in: Nucleic acids research, Vol. 37, Issue 5, pp. 1650-62, (2009) (PubMed).

Zhao, Pellenz, Stoddard: "Activity and specificity of the bacterial PD-(D/E)XK homing endonuclease I-Ssp6803I." in: Journal of molecular biology, Vol. 385, Issue 5, pp. 1498-510, (2009) (PubMed).

Pellenz, Harington, Dujon, Wolf, Schäfer: "Characterization of the I-Spom I endonuclease from fission yeast: insights into the evolution of a group I intron-encoded homing endonuclease." in: Journal of molecular evolution, Vol. 55, Issue 3, pp. 302-13, (2003) (PubMed).

McConnell Smith, Takeuchi, Pellenz, Davis, Maizels, Monnat, Stoddard: "Generation of a nicking enzyme that stimulates site-specific gene conversion from the I-AniI LAGLIDADG homing endonuclease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 13, pp. 5099-104, (2009) (PubMed).

Dujon, Sherman, Fischer, Durrens, Casaregola, Lafontaine, De Montigny, Marck, Neuvéglise, Talla, Goffard, Frangeul, Aigle, Anthouard, Babour, Barbe, Barnay, Blanchin, Beckerich, Beyne, Bleykasten, Boisramé, Boyer, Cattolico, Confanioleri, De Daruvar, Desp: "Genome evolution in yeasts." in: Nature, Vol. 430, Issue 6995, pp. 35-44, (2004) (PubMed).

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