Rabbit anti-Golden Syrian Hamster IgG (F(ab')2 Region) Antibody

Catalog No. ABIN101390

Supplier Product No.: 607-4104

Quick Overview for Rabbit anti-Golden Syrian Hamster IgG (F(ab')2 Region) Antibody (ABIN101390)

Target: IgG

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: F(ab')2 Region

Reactivity: Golden Syrian Hamster

Host: Rabbit

Supplier Product No.: 607-4104

Supplier: Rockland

Purpose: Golden Syrian Hamster IgG F(ab')2 Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Hamster IgG, Hamster IgG F(ab')2, and Hamster Serum.  No reaction was observed against Hamster IgG F(c).

Characteristics: Anti-Golden Syrian Hamster IgG Antibody generated in rabbit detects Golden Syrian Hamster IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose beads.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Hamster IgG F(ab')2 fragment

Application Details

Application Notes: Application Note: Anti-Golden Syrian Hamster IgG F(ab')2 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Golden Syrian Hamster IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Golden Syrian Hamster IgG F(c) will not bind Golden Syrian Hamster IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.