Rabbit anti-Golden Syrian Hamster IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN101391

Supplier Product No.: 607-4504

Quick Overview for Rabbit anti-Golden Syrian Hamster IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP)) (ABIN101391)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: F(ab')2 Region

Reactivity: Golden Syrian Hamster

Host: Rabbit

Supplier Product No.: 607-4504

Supplier: Rockland

Purpose: Golden Syrian Hamster IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Hamster IgG and Hamster Serum.   No reaction was observed against Hamster IgG F(c).

Characteristics: Anti-Golden Syrian Hamster IgG Alkaline Phosphatase Antibody generated in rabbit detects Golden Syrian Hamster IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Hamster IgG F(ab')2 fragment

Application Details

Application Notes: Application Note: This product has been assayed against 1.0 μg of Hamster IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.  Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.8 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Golden Syrian Hamster IgG F(ab')2 Alkaline Phosphatase Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Golden Syrian Hamster IgG F(c) will not bind Golden Syrian Hamster IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.