Goat anti-Mouse IgG1 (Heavy Chain) Antibody (FITC)

Catalog No. ABIN1967635

Quick Overview for Goat anti-Mouse IgG1 (Heavy Chain) Antibody (FITC) (ABIN1967635)

Target: IgG1

Clonality: Polyclonal

Conjugate: FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), FLISA

Product Details

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 0.5 % of target signal.

Purification: Immunoaffinity purified

Immunogen: Mouse IgG heavy chain.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: FLISA (1:10000 - 1:50000), Flo, IF (1:1000 - 1:5000), IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Mouse IgG1 FITC conjugated secondary antibody reagents are ideal for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy as well as other antibody detection methods. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details

Target: IgG1

Target Type: Antibody