Goat anti-Mouse Ig Antibody (Biotin) - Preadsorbed

Catalog No. ABIN2689598

Quick Overview for Goat anti-Mouse Ig Antibody (Biotin) - Preadsorbed (ABIN2689598)

Target: Ig

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Flow Cytometry (FACS)

Isotype: Ig Fraction

Product Details

Reactivity: Mouse

Host: Goat

Characteristics: This polyclonal antibody reacts with whole molecule mouse IgG (heavy and light chains) and may also react with the light chains of other mouse immunoglobulins. This antibody has been reported to be reactive in ELISA with mouse IgG, IgM, and IgA. In addition, low level cross-reactivity with rat and hamseter Ig has been reported. Investigators should note that reactivity with some mouse IgM antibodies can be weak. Minimal cross-reaction to rat, human, bovine, horse and rabbit non-immunoglobulin serum proteins are expected. Note: This reagent may have considerable cross-reactivity on human peripheral blood leukocytes, contributing to undesired background signal, when used with the flow cytometric application. As a result, this product is not recommended for immunofluorescent staining and flow cytometric analysis of human cells. For this application, investigators may wish to consider FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988) as an alternative. For immunohistochemical staining, Biotin Goat Anti-Mouse Ig (Cat. No. 550337) is recommended.

BD Pharmingen™ Biotin Goat Anti-Mouse Ig (Multiple Adsorption) - Biotin - Clone Polyclonal - Isotype Goat Ig - Reactivity Ms - 0.5 mg

Purification: Preadsorption: Negative adsorption

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Target Details

Target: Ig