Rabbit anti-Mouse IgG1 Antibody (FITC)

Catalog No. ABIN339328

Quick Overview for Rabbit anti-Mouse IgG1 Antibody (FITC) (ABIN339328)

Target: IgG1

Clonality: Polyclonal

Conjugate: FITC

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Rabbit

Specificity: Recognizes mouse IgG1 heavy chain. Cross-reactivity against other mouse or human heavy or light chain isotypes is < 1 % . Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgG and mouse Serum.

Purification: Immunoaffinity purified

Immunogen: Mouse IgG1 gamma 1 chain.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA, Flo (1:2000 - 1:10000), IF (1:500 - 1:2500), WB

Usage: Suitable for use in ELISA, Immunomicroscopy, Flow Cytometry, FACS analysis and fluorescent Western Blot. Also suitable for multiplex analysis. Immunomicroscopy: 1:500-1:2500. Flow Cytometry: 1:2000-1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1 mL sterile ddH20

Concentration: Lot specific

Buffer: Lyophilized powder from PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.15M sodium chloride, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store lyophilized powder -20°C. Reconstitute with sterile ddH20, aliquot and store at -20°C.

Target Details

Target: IgG1

Target Type: Antibody