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Rat anti-Mouse IgG1 Antibody (Biotin)

ELISA, FACS Monoclonal SB77e IgG2b Biotin
Catalog No. ABIN375927

Quick Overview for Rat anti-Mouse IgG1 Antibody (Biotin) (ABIN375927)

Target

IgG1

Clonality

  • 95
  • 63
Monoclonal

Conjugate

  • 53
  • 20
  • 20
  • 18
  • 11
  • 8
  • 7
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Biotin

Application

  • 88
  • 66
  • 52
  • 50
  • 30
  • 25
  • 19
  • 16
  • 14
  • 13
  • 11
  • 10
  • 9
  • 8
  • 4
  • 3
  • 2
  • 2
  • 2
ELISA, Flow Cytometry (FACS)

Clone

SB77e

Isotype

IgG2b
  • Reactivity

    • 96
    • 28
    • 24
    • 9
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    Mouse

    Host

    • 60
    • 45
    • 43
    • 14
    • 10
    • 6
    • 1
    Rat

    Specificity

    Mouse IgG1 Fc Minimal reactivity to human, goat, and rabbit IgG

    Characteristics

    Rat Anti-Mouse IgG1-BIOT

    Purification

    Purified

    Immunogen

    Unknown
  • Application Notes

    • Applications: FC - Quality tested , ELISA - Quality tested , FLISA - Quality tested IHC-FS - Reported in literature , WB - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1.0 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg in 1.0 mL of PBS/Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid cycles of freezing and thawing.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Target

    IgG1

    Target Type

    Antibody
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