Goat anti-Mouse IgG2b Antibody (HRP) - Preadsorbed

Catalog No. ABIN376251

Quick Overview for Goat anti-Mouse IgG2b Antibody (HRP) - Preadsorbed (ABIN376251)

Target: IgG2b

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISpot

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Purpose: Goat Anti-Mouse IgG2b, Human ads-HRP

Specificity: Reacts with the heavy chain of mouse IgG 2b as demonstrated by ELISA and flow cytometry.

Characteristics: Goat Anti-Mouse IgG2b-HRP antibody with minimal reactivity to human proteins for use in ELISA, immunohistochemistry / immunocytochemistry, western blotting, and ELISpot assays.

Purification:

Purified by affinity chromatography on mouse IgG2b covalently linked to agarose, cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA / human, bovine, and horse serum proteins (SP) for minimal reactivity

Preadsorption: Human pre-adsorbed

Immunogen: Mouse IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with mouse IgG2b

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 % Glycerol/50 % Phosphate buffered saline, pH 7.4

Storage: 4 °C

Storage Comment: 2-8°C

References

Jin, Nesbitt, Yang, Chen, Horowitz, Jones, Vandergaast, Carey, Reiter, Russell, Kyratsous, Hooper, Hamilton, Ferreira, Deng, Straus, Baras, Hillyer, Luchsinger: "Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and "Reopening"." in: PloS one, Vol. 16, Issue 4, pp. e0250319, (2021) (PubMed).

Nesbitt, Jin, Hogan, Yang, Chen, Chan, Simon, Vargas, King, Huard, Bandy, Hillyer, Luchsinger: "Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats." in: BMC infectious diseases, Vol. 21, Issue 1, pp. 871, (2021) (PubMed).

Luchsinger, Ransegnola, Jin, Muecksch, Weisblum, Bao, George, Rodriguez, Tricoche, Schmidt, Gao, Jawahar, Pal, Schnall, Zhang, Strauss, Yazdanbakhsh, Hillyer, Bieniasz, Hatziioannou: "Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID19 Patients." in: Journal of clinical microbiology, (2020) (PubMed).

Target Details

Target: IgG2b

Target Type: Antibody

Background:

Description: Goat Anti-Mouse IgG2b, Human ads-HRP

NCBI_Official_Symbol: Ighg2b

Gene ID: 16016

UniProt: P01867