Goat anti-Mouse IgG2b Antibody (TRITC) - Preadsorbed

Catalog No. ABIN376754

Quick Overview for Goat anti-Mouse IgG2b Antibody (TRITC) - Preadsorbed (ABIN376754)

Target: IgG2b

Clonality: Polyclonal

Conjugate: TRITC

Application: FLISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Purpose: Goat Anti-Mouse IgG2b, Human ads-TRITC

Specificity: Reacts with the heavy chain of mouse IgG 2b as demonstrated by ELISA and flow cytometry.

Characteristics: Goat Anti-Mouse IgG2b-TRITC antibody with minimal reactivity to human proteins for use in immunohistochemistry / immunocytochemistry assays.

Purification:

Purified by affinity chromatography on mouse IgG2b covalently linked to agarose, cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA / human, bovine, and horse serum proteins (SP) for minimal reactivity

Preadsorption: Human pre-adsorbed

Immunogen: Mouse IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with mouse IgG2b

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing < 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References

Song, Dohn, Rydeen, Nechiporuk, Waxman: "HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development." in: PLoS genetics, Vol. 15, Issue 5, pp. e1008165, (2019) (PubMed).

Rydeen, Waxman: "Cyp26 Enzymes Facilitate Second Heart Field Progenitor Addition and Maintenance of Ventricular Integrity." in: PLoS biology, Vol. 14, Issue 11, pp. e2000504, (2017) (PubMed).

Menzl, Lebeau, Pandey, Hassounah, Li, Nagle, Weihs, McDermott: "Loss of primary cilia occurs early in breast cancer development." in: Cilia, Vol. 3, pp. 7, (2014) (PubMed).

Hassounah, Nagle, Saboda, Roe, Dalkin, McDermott: "Primary cilia are lost in preinvasive and invasive prostate cancer." in: PLoS ONE, Vol. 8, Issue 7, pp. e68521, (2013) (PubMed).

Lionnet, Czaplinski, Darzacq, Shav-Tal, Wells, Chao, Park, de Turris, Lopez-Jones, Singer: "A transgenic mouse for in vivo detection of endogenous labeled mRNA." in: Nature methods, Vol. 8, Issue 2, pp. 165-70, (2011) (PubMed).

Hardy, Filer, Cooper, Parsonage, Raza, Hardie, Rabbitt, Stewart, Buckley, Hewison: "Differential expression, function and response to inflammatory stimuli of 11beta-hydroxysteroid dehydrogenase type 1 in human fibroblasts: a mechanism for tissue-specific regulation of inflammation." in: Arthritis research & therapy, Vol. 8, Issue 4, pp. R108, (2007) (PubMed).

Target Details

Target: IgG2b

Target Type: Antibody

Background:

Description: Goat Anti-Mouse IgG2b, Human ads-TRITC

NCBI_Official_Symbol: Ighg2b

Gene ID: 16016

UniProt: P01867