Mouse anti-Human IgA2 Antibody

Catalog No. ABIN457823

Quick Overview for Mouse anti-Human IgA2 Antibody (ABIN457823)

Target: IgA2

Clonality: Monoclonal

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Haemagglutination (H)

Clone: NI 512 (A89-038)

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: The reactivity of the antiserum is restricted to subclass specific determinants on the IgA2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Purified monoclonal mouse antibody to human IgA2, sub-class specific

Characteristics: Purified monoclonal mouse antibody to human IgA2, sub-class specific

Purification: Purified

Immunogen: Highly purified monoclonal IgA2 isolated from human serum.

Application Details

Application Notes: To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgA2, haemagglutination and immunoblotting.
The optimum working dilu-tion is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2)

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgA2