Rabbit anti-Mouse IgG1 Antibody - Preadsorbed

Catalog No. ABIN458529

Product Details

Target: IgG1

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: The defined antibody specificity is directed to IgG1 as tested against mouse sera and a panel of purified homogenous immunoglobulins and at the level of sensitivity of direct immunofluorescence staining methods applied to lymphoid cells with the conjugated form of this product. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against mouse serum and the purified monoclonal IgG1

Characteristics: Purified IgG fraction of polyclonal rabbit antiserum to mouse IgG1, subclass specific
Performance testing Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG(7S) is further evaluated in a number of highly sensitive non-precipitating antibody-binding as

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogen: Pools of highly purified homogenous IgG1 isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: The cytochemical grade of RAM/IgG1/7S allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted.
The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:5,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

References

Kim, Cao, Finkelstein, Cárdenas, Erickson, Mehta: "A two-colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line." in: Malaria journal, Vol. 18, Issue 1, pp. 313, (2019) (PubMed).

Target Details

Target: IgG1

Abstract: IgG1 Products

Target Type: Antibody