Goat anti-Human IgA, IgG, IgM Antibody (HRP)

Catalog No. ABIN488655

Quick Overview for Goat anti-Human IgA, IgG, IgM Antibody (HRP) (ABIN488655)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Reactivity: Human

Host: Goat

Specificity: Human IgA+IgG+IgM heavy chains and light chains on all Human immunoglobulins.

Predicted Reactivity: human IgA+IgG+IgM heavy chains and light chains on all human immunoglobulins

Characteristics: Goat anti-human IgA+IgG+IgM is a secondary antibody conjugated to HRP which binds to human immunoglobulins in immunological assays.

Purification: Affinity purification

Immunogen: affinity purified human IgA+IgG+IgM

Application Details

Application Notes: 1: 10 000 -1: 50 000 (ELISA), (WB), 1: 500 -1: 5000 (IHC)

Comment:

No reactivity to non immunoglobulin human proteins based on immunoelectrophoresis

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Buffer: 10 mM Sodium Phosphate, 0.15 M SodiumChloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.

Preservative: Kathon CG

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C

Storage Comment: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgA+IgG+IgM

Target Type: Antibody