Goat anti-Human IgA (Free & Bound), (Secretory Component) Antibody (Biotin)
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- Target See all IgA products
- IgA
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Binding Specificity
- Free & Bound, Secretory Component
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunodiffusion (ID), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB)
- Characteristics
- Molar Ratio: Biotin/IgG aprox. 4.5
- Purification
- Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and purification of the IgG fraction by DEAE-Chromatography.
- Immunogen
- Purified free Human Secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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Goat anti-Human IgA (Heavy Chain) antibody (Biotin)
ELISA, WB, FACS Polyclonal IgG Biotin
Rabbit anti-Human IgA (Heavy Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgA (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Human IgA (Heavy Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Human IgA (Heavy Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Restore by adding 1.0 mL of sterile distilled water.
- Concentration
- 10.0 mg/mL
- Buffer
- PBS, pH 7.2 without preservatives and foreign proteins
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -28 °C for longer.
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- Target
- IgA
- Abstract
- IgA Products
- Target Type
- Antibody
- Background
- Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM.Synonyms: Human Immunoglobulin A
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