Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN619116

Quick Overview for Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN619116)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Donkey

Purpose: Donkey anti-Goat IgG (H&L), ALP conjugated

Purification: Immunogen affinity purified

Application Details

Application Notes: 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunogen affinity purified IgG.

Handling Advice: Prepare working dilution prior to use and discard after use. Do not store working dilution,Be sure to mix well but without foaming

Storage: 4 °C,-20 °C

Storage Comment: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Expiry Date: 48 months

Target Details

Target: IgG

Target Type: Antibody

Background:

Background: Donkey anti-goat IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.

Additional information: This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.