Mouse anti-Human Immunoglobulin kappa Chain Complex (Igk) Antibody

Catalog No. ABIN7881161

Quick Overview for Mouse anti-Human Immunoglobulin kappa Chain Complex (Igk) Antibody (ABIN7881161)

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Clonality: Monoclonal

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM558

Isotype: IgG1, kappa

Product Details

Reactivity: Human

Host: Mouse

Purpose: Anti-Kappa Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human Ig kappa chain was used as the immunogen for this anti-Kappa antibody.

Application Details

Application Notes: The optimal dilution of the anti-Kappa antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Kappa antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Alternative Name: Kappa

Background: This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

UniProt: P01601