anti-Mouse (Murine) CD80 antibody for Flow Cytometry

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • CD80 molecule
  • Cd80 molecule
  • CD80 antigen
  • CD80
  • Cd80
Epitope
AA 37-245
33
24
19
15
15
15
14
11
10
10
8
7
6
5
5
5
4
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
496
293
49
11
11
6
3
3
3
2
1
1
Host
Goat
441
125
99
94
47
16
Clonality
Polyclonal
Conjugate
This CD80 antibody is un-conjugated
76
75
56
44
21
19
19
17
10
9
9
7
7
7
7
7
7
6
6
6
6
6
5
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
554
221
171
158
119
100
81
41
41
26
23
21
12
8
7
6
5
5
5
5
4
2
2
2
2
1
1
1
Supplier
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Catalog No. ABIN4900307
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.728527 ABIN2665750 FACS Hamster IgG Log in to see 16-10A1 11
13.728527 ABIN2659751 BR CyTOF Dep FACS IHC IP PE/Dazzle™ 594 Hamster IgG Log in to see 16-10A1 11
13.728527 ABIN2663589 FACS PE Hamster IgG Log in to see 16-10A1 11
13.728527 ABIN2661133 FACS Biotin Hamster IgG Log in to see 16-10A1 11
13.728527 ABIN2662310 FACS IF IHC Pacific Blue Hamster IgG Log in to see 16-10A1 11
13.728527 ABIN2660414 FACS PerCP-Cy5.5 Hamster IgG Log in to see 16-10A1 11
13.728527 ABIN2659319 FACS PE-Cy7 Hamster IgG Log in to see 16-10A1 11
13.728527 ABIN2657845 CyTOF FACS IHC Alexa Fluor 647 Hamster IgG Log in to see 16-10A1 11
13.728527 ABIN371005 FACS FITC Rat IgG2a Log in to see 1G10 5
13.728527 ABIN2664828 FACS Hamster IgG Log in to see 16-10A1 11
13.728527 ABIN2664829 CyTOF FACS Hamster IgG Log in to see 16-10A1 11
13.728527 ABIN1176972 BR IHC (fro) FACS IP Armenian Hamster IgG2, kappa Log in to see 16-10A1 6
13.728527 ABIN951413 FACS Rat IgG2a Log in to see RMMP-1 1
13.728527 ABIN440010 FACS in vitro IP PE Rat IgG2a kappa Log in to see 1G10 0
13.728527 ABIN440011 FACS in vitro IP APC Rat IgG2a kappa Log in to see 1G10 0
13.728527 ABIN440009 FACS in vitro IP Biotin Rat IgG2a kappa Log in to see 1G10 0
13.728527 ABIN440008 FACS in vitro IP FITC Rat IgG2a kappa Log in to see 1G10 0
1 ABIN489934 Func FACS IHC (fro) IP Hamster IgG2 Log in to see 16-10A1 8
1 ABIN2689407 BR IHC (fro) FACS IP Armenian Hamster IgG2, kappa Log in to see 16 6
1 ABIN2689413 FACS Biotin Armenian Hamster IgG2, kappa Log in to see 16 5

General

Antigen CD80 Antibodies
Epitope AA 37-245
(33), (24), (19), (15), (15), (15), (14), (11), (10), (10), (8), (7), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(496), (293), (49), (11), (11), (6), (3), (3), (3), (2), (1), (1)
Host Goat
(441), (125), (99), (94), (47), (16)
Clonality
Polyclonal
Conjugate This CD80 antibody is un-conjugated
(76), (75), (56), (44), (21), (19), (19), (17), (10), (9), (9), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
(554), (221), (171), (158), (119), (100), (81), (41), (41), (26), (23), (21), (12), (8), (7), (6), (5), (5), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN4900307) Images
Specificity Detects mouse B7-1/CD80 in ELISAs and Western blots.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse B7-1/CD80, Asp37-Lys245, Accession # Q00609
Isotype IgG
Plasmids, Primers & others

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 antibody (ABIN4900307) Images back to top
Antigen
Alternative Name B7-1/CD80 (CD80 Antibody Abstract)
Background Alternative Names: Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 Molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Gene ID 941
UniProt Q00609
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 antibody (ABIN4900307) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Neutralization 0.15-0.6 μg/mL
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 antibody (ABIN4900307) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD80 antibody (ABIN4900307)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Product cited in:

Garg, Delaney, Toubai, Ghosh, Reddy, Banerjee, Yung: "Aging is associated with increased regulatory T-cell function." in: Aging cell, Vol. 13, Issue 3, pp. 441-8, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Sofra, Mansour, Liu, Gao, Primpidou, Wang, Li: "Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection." in: European journal of immunology, Vol. 39, Issue 1, pp. 85-95, 2009 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

He, Li, Chen, Zhang, Chen, Hayashida, Zhu, Tseng: "Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract." in: Investigative ophthalmology & visual science, Vol. 49, Issue 10, pp. 4468-75, 2008 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN4900307) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of B7-1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes eith...
Western Blotting (WB) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of Mouse B7-1/CD80 by Western Blot. Western blot shows lysates of C2C12 mou...
Neutralization (Neut) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) IL-2 secretion Induced by B7-1/CD80 and Neutralization by Mouse B7-1/CD80 Antibody. R...
Immunocytochemistry (ICC) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) anti-CD80 (CD80) (AA 37-245) antibody (Image 4)