anti-Mouse (Murine) CD80 antibody for Flow Cytometry

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • CD80 molecule
  • Cd80 molecule
  • CD80 antigen
  • CD80
  • Cd80
Epitope
AA 37-245
33
24
15
15
15
14
12
11
11
10
8
7
6
5
5
5
4
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
456
285
49
11
11
6
3
3
2
2
1
Host
Goat
399
126
97
94
42
15
Clonality
Polyclonal
Conjugate
This CD80 antibody is un-conjugated
71
70
53
40
19
17
16
15
10
7
7
6
6
6
6
6
6
6
6
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
508
219
171
153
115
101
81
41
40
26
22
22
11
8
7
6
5
5
5
5
2
2
2
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN4900307
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.572235 ABIN489934 Func FACS IHC (fro) IP Hamster IgG2 Log in to see 16-10A1 8
18.36386 ABIN1176972 BR IHC (fro) FACS IP Armenian Hamster IgG2, kappa Log in to see 16-10A1 6
15.572235 ABIN135271 FACS PE Rat IgG2a Log in to see 1G10 3
15.572235 ABIN135269 FACS FITC Rat IgG2a Log in to see 1G10 3
15.572235 ABIN135270 FACS Biotin Rat IgG2a Log in to see 1G10 3
15.572235 ABIN135272 FACS Rat IgG2a Log in to see 1G10 3
15.572235 ABIN135268 FACS Rat IgG2a Log in to see 1G10 3
15.572235 ABIN375569 FACS SPRD Rat IgG2a Log in to see 1G10 0
15.572235 ABIN375397 FACS APC Rat IgG2a Log in to see 1G10 0
15.572235 ABIN375521 FACS PE Rat IgG2a Log in to see 1G10 0
12.572235 ABIN6150609 FACS PerCP Armenian Hamster IgG2 Log in to see 16-10A1 0
12.572235 ABIN6150616 FACS PerCP-Cy5.5 Armenian Hamster IgG2 Log in to see 16-10A1 0
12.572235 ABIN6150605 FACS FITC Armenian Hamster IgG2 Log in to see 16-10A1 0
12.572235 ABIN6150608 FACS PerCP Armenian Hamster IgG2 Log in to see 16-10A1 0
12.572235 ABIN6150603 FACS FITC Armenian Hamster IgG2 Log in to see 16-10A1 0
12.572235 ABIN6150607 FACS PE Armenian Hamster IgG2 Log in to see 16-10A1 0
12.572235 ABIN6150606 FACS PE Armenian Hamster IgG2 Log in to see 16-10A1 0
12.572235 ABIN6150604 FACS FITC Armenian Hamster IgG2 Log in to see 16-10A1 0
12.572235 ABIN6150610 FACS APC Armenian Hamster IgG2 Log in to see 16-10A1 0
12.572235 ABIN6150611 FACS APC Armenian Hamster IgG2 Log in to see 16-10A1 0

General

Antigen CD80 Antibodies
Epitope AA 37-245
(33), (24), (15), (15), (15), (14), (12), (11), (11), (10), (8), (7), (6), (5), (5), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(456), (285), (49), (11), (11), (6), (3), (3), (2), (2), (1)
Host Goat
(399), (126), (97), (94), (42), (15)
Clonality
Polyclonal
Conjugate This CD80 antibody is un-conjugated
(71), (70), (53), (40), (19), (17), (16), (15), (10), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
(508), (219), (171), (153), (115), (101), (81), (41), (40), (26), (22), (22), (11), (8), (7), (6), (5), (5), (5), (5), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN4900307) Images
Specificity Detects mouse B7-1/CD80 in ELISAs and Western blots.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse B7-1/CD80, Asp37-Lys245, Accession # Q00609
Isotype IgG
Plasmids, Primers & others

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 antibody (ABIN4900307) Images back to top
Antigen
Alternative Name B7-1/CD80 (CD80 Antibody Abstract)
Background Alternative Names: Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 Molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Gene ID 941
UniProt Q00609
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 antibody (ABIN4900307) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Neutralization 0.15-0.6 μg/mL
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 antibody (ABIN4900307) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD80 antibody (ABIN4900307)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Product cited in:

Clements, Long, Long, Figley, Smith, Ostrand-Rosenberg: "Frontline Science: High fat diet and leptin promote tumor progression by inducing myeloid-derived suppressor cells." in: Journal of leukocyte biology, Vol. 103, Issue 3, pp. 395-407, 2018 Method employed by authors: Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Garg, Delaney, Toubai, Ghosh, Reddy, Banerjee, Yung: "Aging is associated with increased regulatory T-cell function." in: Aging cell, Vol. 13, Issue 3, pp. 441-8, 2015 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Sofra, Mansour, Liu, Gao, Primpidou, Wang, Li: "Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection." in: European journal of immunology, Vol. 39, Issue 1, pp. 85-95, 2009 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

He, Li, Chen, Zhang, Chen, Hayashida, Zhu, Tseng: "Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract." in: Investigative ophthalmology & visual science, Vol. 49, Issue 10, pp. 4468-75, 2008 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN4900307) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of B7-1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes eith...
Western Blotting (WB) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of Mouse B7-1/CD80 by Western Blot. Western blot shows lysates of C2C12 mou...
Neutralization (Neut) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) IL-2 secretion Induced by B7-1/CD80 and Neutralization by Mouse B7-1/CD80 Antibody. R...
Immunocytochemistry (ICC) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) anti-CD80 (CD80) (AA 37-245) antibody (Image 4)