Quick Overview for Human Normal Tissue Blot I (ABIN2455962)
Host
Application
-
-
Characteristics
-
The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: brain, 3: colon, 4: heart, 5: kidney, 6: liver, 7: lung, 8: pancreas, and 9: spleen.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
-
-
Rat Tail
Host: Rat
Guinea Pig Spinal CordHost: Guinea Pig Normal Spinal Cord
Sheep Red Blood Cells (10% Washed Pooled Cells)H Host: Sheep Normal Red Blood Cells
Mouse BrainIEM, IHC, WB Host: Mouse Normal Brain
Chicken Red Blood Cells (10% Washed Pooled Cells)H Host: Chicken Normal Red Blood Cells
Hamster Complement (Lyophilized) With DiluentHost: Hamster Normal Complement
Guinea Pig Complement (Lyophilized) With DiluentHost: Guinea Pig Normal Complement
Rabbit Blood (In Sodium Citrate)H Host: Rabbit Normal Blood
Sheep Red Blood Cells (100% Washed Pooled Cells)H Host: Sheep Normal Red Blood Cells
-
-
Application Notes
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
-
Restrictions
- For Research Use only
-
-
-
Handling Advice
- The blot can be reused after stripping.
-
Storage
- 4 °C
-
Storage Comment
- Store at 4 °C.
-
-
