Human Normal Tissue Blot II
WB
Host: Human
Catalog No. ABIN2455963
$488.40
Plus shipping costs $50.00
1 applications
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Quick Overview for Human Normal Tissue Blot II (ABIN2455963)
Host
Human
Application
Western Blotting (WB)
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Characteristics
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, and spleen.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: brain, 3: heart, 4: kidney, 5: liver, 6: lung, 7: pancreas, 8: skeletal muscle, 9: spleen, and 10: skin.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Application Notes
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
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Restrictions
- For Research Use only
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Handling Advice
- The blot can be reused after stripping.
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Storage
- 4 °C
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Storage Comment
- Store at 4 °C.
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