Human Normal Tissue Blot IV
Quick Overview for Human Normal Tissue Blot IV (ABIN2455965)
Host
Application
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Characteristics
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are bladder, breast, cervix, kidney, ovary, placenta, prostate, testis, and uterus.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: bladder, 3: breast, 4: cervix, 5: kidney, 6: ovary, 7: placenta, 8: prostate, 9: testis, and 10: uterus
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Application Notes
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
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Restrictions
- For Research Use only
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Handling Advice
- The blot can be reused after stripping.
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Storage
- 4 °C
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Storage Comment
- Store at 4 °C.
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