Human Normal Tissue Blot V
Quick Overview for Human Normal Tissue Blot V (ABIN2455966)
Host
Application
-
-
Characteristics
-
The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are appendix, kidney, liver, lymph node, peripheral blood leukocyte, spleen, thymus, tonsil, and thyroid.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: appendix, 3: kidney, 4: liver, 5: lymph node, 6: peripheral blood leukocyte, 7: spleen, 8: thymus, 9: tonsil, and 10: thyroid
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
-
-
Sheep Red Blood Cell (RBC) 10% Washed Pooled Cells
CA, FACS Host: Sheep Blood
Guinea Pig Spinal CordHost: Guinea Pig Normal Spinal Cord
Hamster Complement (Lyophilized) with DiluentHost: Hamster Complement
Guinea Pig Complement (Lyophilized) with DiluentELISA Host: Guinea Pig Complement
Guinea Pig Complement (Fresh Frozen)CA, ELISA, FACS Host: Guinea Pig Complement
Rabbit Blood in Sodium CitrateH Host: Rabbit Blood
Chicken Red Blood Cells (RBC) 10% Washed Pooled CellsChIP Host: Chicken Blood
Human Red Blood Cells (RBC) 10% Washed Pooled CellsHost: Human Blood
Sheep Red Blood Cell (RBC) 100% Washed Pooled CellsFunc, IF Host: Sheep Blood
-
-
Application Notes
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
-
Restrictions
- For Research Use only
-
-
-
Handling Advice
- The blot can be reused after stripping.
-
Storage
- 4 °C
-
Storage Comment
- Store at 4 °C.
-
-
