Human Normal Tissue Blot VI
WB
Host: Human
Catalog No. ABIN2455967
$488.40
Plus shipping costs $50.00
1 applications
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Quick Overview for Human Normal Tissue Blot VI (ABIN2455967)
Host
Human
Application
Western Blotting (WB)
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Characteristics
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: left cerebellum, 3: right cerebellum, 4: frontal lobe, 5: occipital lobe, 6: parietal lobe, 7: whole eye, 8: spinal cord, 9: temporal lobe, and 10: thalamus.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Application Notes
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
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Restrictions
- For Research Use only
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Handling Advice
- The blot can be reused after stripping.
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Storage
- 4 °C
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Storage Comment
- Store at 4 °C.
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