Human Lung Tumor Blot VI
WB
Host: Human
Catalog No. ABIN2455976
$730.40
Plus shipping costs $50.00
1 applications
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Quick Overview for Human Lung Tumor Blot VI (ABIN2455976)
Host
Human
Application
Western Blotting (WB)
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Characteristics
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 1 normal human lung tissue, the cell line A549 (human lung carcinoma), and 7 different human lung tumor tissue lysates with 15 μg of total cellular protein in each lane. Proteins in a wide range of molecular mass are separated in a 4-20 % gradient SDS-PAGE gel and transferred onto nitrocellulose membranes. The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 non-small cell carcinoma, 1 differentiated adenocarcinoma, 1 squamous cell carcinoma, 1 pleomorphic carcinoma and 2 non-diagnosed carcinomas (from different donors).
Sample diagnosis: Tumor
Samples loaded: Lanes: 1: protein markers, 2: A549, 3: normal lung tissue, 4: small cell carcinoma, 5: non-small cell carcinoma, 6: differentiated adenocarcinoma, 7: squamous cell carcinoma, 8: pleo-morphic carcinoma, 9: non-diagnosed carcinoma: 10: non-diagnosed carcinoma.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Application Notes
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
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Restrictions
- For Research Use only
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Handling Advice
- The blot can be reused after stripping.
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Storage
- 4 °C
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Storage Comment
- Store at 4 °C.
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