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anti-Sphingosine Kinase 1 (SPHK1) Antibodies

The protein encoded by SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Additionally we are shipping Sphingosine Kinase 1 Proteins (14) and Sphingosine Kinase 1 Kits (8) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
SPHK1 8877 Q9NYA1
SPHK1 20698 Q8CI15
SPHK1 170897 Q91V26
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Top anti-Sphingosine Kinase 1 Antibodies at antibodies-online.com

Showing 10 out of 195 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Goat Un-conjugated ELISA, WB ABIN308402 (2µg/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. 100 μg Log in to see 4 to 5 Days
$326.89
Details
Human Rabbit Un-conjugated EIA, WB The anti-SphK1 Pab is used in Western blot (Lane 2) to detect c-myc-tagged SphK1 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 1). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH). Western blot analysis of anti-hSPHK1-M1 Pab in 293 cell line lysates transiently transfected with the SPHK1 gene (2 µg/lane). hSPHK1-M1(arrow) was detected using the purified Pab (1:60 dilution). 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB Formalin-fixed and paraffin-embedded human cancer tissue (hepatocarcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. The anti-SPHK1 Pab is used in Western blot to detect SPHK1 in mouse kidney tissue lysate. 0.4 mL Log in to see 6 to 8 Days
$401.50
Details
Human Rabbit Un-conjugated WB The anti-SphK1 Pab (ABIN391345) is used in Western blot (Lane 2) to detect c-myc-tagged SphK1 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 1). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH). Western blot analysis of anti-hSPHK1-M1 Pab (ABIN391345) in 293 cell line lysates transiently transfected with the SPHK1 gene (2 µg/lane). hSPHK1-M1(arrow) was detected using the purified polyclonal antibody (1:60 dilution). 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated IHC (p), WB The anti-SPHK1 Pab (ABIN391346) is used in Western blot to detect SPHK1 in mouse kidney tissue lysate. Western blot analysis of anti-SPHK1 Antibody (Center) (ABIN391346) in 293 cell line lysates (35 µg/lane). SPHK1 (arrow) was detected using the purified polyclonal antibody. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated EIA, WB Western blot analysis of anti-SPHK1 Antibody (N-term) in K562 cell line lysates (35 µg/lane). SPHK1 (arrow) was detected using the purified Pab. 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Goat Un-conjugated EIA, WB 20 μg Log in to see 6 to 8 Days
$203.50
Details
Cow Goat Un-conjugated EIA, IP, WB 0.1 mg Log in to see 6 to 8 Days
$379.50
Details
Mouse Rabbit Un-conjugated WB 0.1 mg Log in to see 10 to 12 Days
$623.70
Details
Human Rabbit Un-conjugated WB Western blot analysis of anti-SPHK1 Antibody (N-term P74)(ABIN391347) in K562 cell line lysates (35 µg/lane). SPHK1(arrow) was detected using the purified polyclonal antibody. Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (N-term P74) (ABIN391347). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene. 400 μL Log in to see 10 to 11 Days
$324.50
Details

SPHK1 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-Sphingosine Kinase 1 Antibodies

  1. Human Polyclonal SPHK1 Primary Antibody for IHC (p), WB - ABIN391346 : Nayak, Huo, Kwang, Pushparaj, Kumar, Ling, Dheen: Sphingosine kinase 1 regulates the expression of proinflammatory cytokines and nitric oxide in activated microglia. in Neuroscience 2010 (PubMed)
    Show all 17 references for ABIN391346

  2. Human Polyclonal SPHK1 Primary Antibody for EIA, IHC (p) - ABIN359438 : Li, Yu, Xia, Zhang, Weng, Zheng, Kong, Hu, Zeng, Zeng, Li, Li, Song: Sphingosine kinase 1 is associated with gastric cancer progression and poor survival of patients. in Clinical cancer research : an official journal of the American Association for Cancer Research 2009 (PubMed)
    Show all 5 references for ABIN359438

  3. Human Polyclonal SPHK1 Primary Antibody for EIA, WB - ABIN359437 : Nava, Lacana, Poulton, Liu, Sugiura, Kono, Milstien, Kohama, Spiegel: Functional characterization of human sphingosine kinase-1. in FEBS letters 2000 (PubMed)
    Show all 5 references for ABIN359437

  4. Human Polyclonal SPHK1 Primary Antibody for EIA, WB - ABIN359439 : Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: Complete sequencing and characterization of 21,243 full-length human cDNAs. ... in Nature genetics 2003 (PubMed)
    Show all 4 references for ABIN359439

  5. Human Polyclonal SPHK1 Primary Antibody for WB - ABIN391347 : Di, Kawamura, Gao, Tang, Berdyshev, Vogel, Zhao, Sharma, Bachmaier, Xu, Malik: A novel function of sphingosine kinase 1 suppression of JNK activity in preventing inflammation and injury. in The Journal of biological chemistry 2010 (PubMed)
    Show all 2 references for ABIN391347

  6. Human Polyclonal SPHK1 Primary Antibody for WB - ABIN391345 : Hayashi, Nakagami, Takami, Koriyama, Mori, Tamai, Sun, Nagao, Morishita, Kaneda: FHL-2 suppresses VEGF-induced phosphatidylinositol 3-kinase/Akt activation via interaction with sphingosine kinase-1. in Arteriosclerosis, thrombosis, and vascular biology 2009 (PubMed)
    Show all 2 references for ABIN391345

  7. Mouse (Murine) Polyclonal SPHK1 Primary Antibody for WB - ABIN4620349 : Kihara, Ikeda, Kariya, Lee, Lee, Igarashi: Sphingosine-1-phosphate lyase is involved in the differentiation of F9 embryonal carcinoma cells to primitive endoderm. in The Journal of biological chemistry 2003 (PubMed)

  8. Human Polyclonal SPHK1 Primary Antibody for EIA, WB - ABIN782886 : Kohama, Olivera, Edsall, Nagiec, Dickson, Spiegel: Molecular cloning and functional characterization of murine sphingosine kinase. in The Journal of biological chemistry 1998 (PubMed)

  9. Human Polyclonal SPHK1 Primary Antibody for ELISA, WB - ABIN308402 : Kase, Hattori, Jojima, Okayasu, Tomizawa, Suzuki, Banba, Monden, Satoh, Akimoto, Kasai: Globular adiponectin induces adhesion molecule expression through the sphingosine kinase pathway in vascular endothelial cells. in Life sciences 2007 (PubMed)

  10. Cow (Bovine) Polyclonal SPHK1 Primary Antibody for EIA, IP - ABIN375022 : Igarashi, Okada, Hayashi, Fujita, Jahangeer, Nakamura: Sphingosine kinase 2 is a nuclear protein and inhibits DNA synthesis. in The Journal of biological chemistry 2003 (PubMed)

More Antibodies against Sphingosine Kinase 1 Interaction Partners

Fruit Fly (Drosophila melanogaster) Sphingosine Kinase 1 (SPHK1) interaction partners

  1. Sk1 and Sk2 (show KCNN2 Antibodies) are not redundant in biological function and are essential for diverse physiological functions in Drosophila

Arabidopsis thaliana Sphingosine Kinase 1 (SPHK1) interaction partners

  1. SPHK/phyto-S1P (show MBTPS1 Antibodies) and PLDalpha1A are co-dependent in amplification of response to ABA, mediating stomatal closure in Arabidopsis.

  2. Sphingosine kinase 1 phosphorylates sphingosine-1-phosphate and phytosphingosine-1-phosphate, and is coordinately regulated with sphingosine-1-phosphate phosphatase.

Rabbit Sphingosine Kinase 1 (SPHK1) interaction partners

  1. Melatonin administration significantly inhibited those changes and induced a decreased immunoreactivity for rabbit hemorrhagic disease virus VP60 antigen in the liver. Results obtained indicate that the SphK1/S1P (show MBTPS1 Antibodies) system activates in parallel to viral replication and the inflammatory process induced by the virus.

Human Sphingosine Kinase 1 (SPHK1) interaction partners

  1. These results highlight the importance of sphingosine and its conversion to sphingosine-1-phosphate by SphK1 in endocytic membrane trafficking.

  2. By simulating therapeutic strategies that target multiple nodes of the pathway such as Raf and SphK1, we conclude that combination therapy should be much more effective in blocking VEGF signaling to EKR1/2. The model has important implications for interventions that target signaling pathways in angiogenesis relevant to cancer, vascular diseases, and wound healing.

  3. SphK1 is regulated by PDGF (show PDGFA Antibodies)-BB in pulmonary artery smooth muscle cells via the transcription factor Egr-1 (show EGR1 Antibodies), promoting cell proliferation.

  4. Melatonin-treated cells also exhibited an inhibition of the SphK1/S1P (show MBTPS1 Antibodies) axis. Antifibrogenic effect of SphK1 inhibition was confirmed by treatment of LX2 cells with PF543. Abrogation of the lipid signaling pathway by the indole reveals novel molecular pathways that may account for the protective effect of melatonin in liver fibrogenesis.

  5. demonstrated the roles played by miR (show MLXIP Antibodies)-144-3p and FN1 (show FN1 Antibodies) in mediating the oncogenic function of SphK1, which enhanced the understanding of the etiology of PTC (show F9 Antibodies)

  6. SphK1 is associated with the proliferation and invasiveness of CRC (show CALR Antibodies) cells and the SphK1 gene may contribute to a novel therapeutic approach against CRC (show CALR Antibodies).

  7. The levels of SphK1 were significantly increased in triple-negative breast cancer patients compared with normal controls. SphK1 knockdown with siRNA decreased TNBC cell proliferation and inhibited cell migration/invasion, suggesting that SphK1 has an important role in TNBC.

  8. SPHK1 mRNA is up-regulation in colorectal cancer.

  9. Found that upregulation of SphK1 expression in the stiff substrate is dominant in metastatic cancer cells but not in primary cancer cells. These results suggest that alterations in the mechanical environment of the extracellular matrix surrounding the tumor cells actively regulate cellular properties such as secretion, which in turn, may contribute to cancer progression.

  10. In conclusion, these data suggested that HuR (show ELAVL1 Antibodies) bound to SphK1 mRNA and played a crucial role in TGF-beta1 (show TGFB1 Antibodies)-induced hepatic stellate cell activation.

Mouse (Murine) Sphingosine Kinase 1 (SPHK1) interaction partners

  1. These results highlight the importance of sphingosine and its conversion to sphingosine-1-phosphate by SphK1 in endocytic membrane trafficking.

  2. Melatonin-treated cells also exhibited an inhibition of the SphK1/S1P (show S1PR1 Antibodies) axis. Antifibrogenic effect of SphK1 inhibition was confirmed by treatment of LX2 cells with PF543. Abrogation of the lipid signaling pathway by the indole reveals novel molecular pathways that may account for the protective effect of melatonin in liver fibrogenesis.

  3. These findings suggest that the inhibitory effect of these plant extracts on the activation of AGEs/RAGE (show AGER Antibodies)/SphK1 signaling pathway in db/db (show LEPR Antibodies) diabetic mice kidney is a novel mechanism by which they exert renoprotective effects in diabetic nephropathy.

  4. Keratinocyte-specific deletion of Traf2 (show TRAF2 Antibodies), but not Sphk1 deficiency, disrupted TNF (show TNF Antibodies) mediated NF-kappaB (show NFKB1 Antibodies) and MAP kinase (show MAPK1 Antibodies) signalling and caused epidermal hyperplasia and psoriatic skin inflammation.

  5. SphK1 dependent PKC-delta (show PKCd Antibodies) activation plays an important role in promoting NF-kappaB (show NFKB1 Antibodies) activation and inflammatory response in acute liver failure, and inhibition of PKC-delta (show PKCd Antibodies) activation might be a potential therapeutic strategy for this disease.

  6. Inhibition of SphK1 ameliorates drug-induced acute liver failure by reducing HMGB1 (show HMGB1 Antibodies) cytoplasmic translocation in liver cells.

  7. Collectively, our results demonstrate that PF-543 exerts potent anti-CRC (show SCRIB Antibodies) activity in vitro and in vivo.

  8. transient receptor potential channel 1 suppression of basal sphingosine kinase 1 activity enables endothelial cell-barrier destabilization by edemagenic agonists

  9. Based on these findings, the authors propose new roles for SK1 in affecting innate responses that regulate susceptibility to dengue virus infection.

  10. Together, these results suggest that alpha-MSH alleviates Dex-induced damages to cultured osteoblasts through activating MC4R (show MC4R Antibodies)-SphK1 signaling.

Sphingosine Kinase 1 (SPHK1) Antigen Profile

Protein Summary

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene names and symbols associated with SPHK1

  • Sphingosine kinase 1 (Sk1) antibody
  • sphingosine kinase 1 (SPHK1) antibody
  • sphingosine kinase 1 (sphk1) antibody
  • sphingosine kinase 1 (LOC100357790) antibody
  • sphingosine kinase 1 (Sphk1) antibody
  • 1110006G24Rik antibody
  • CG1747 antibody
  • Dmel\\CG1747 antibody
  • F18E5.160 antibody
  • F18E5_160 antibody
  • Sk1 antibody
  • sphingosine kinase 1 antibody
  • SPHK antibody
  • SPHK1 antibody
  • Spk1 antibody

Protein level used designations for SPHK1

CG1747-PA , CG1747-PB , Sk1-PA , Sk1-PB , sphingosine kinase 1 , sphingosine kinase I , SK 1 , SPK 1

GENE ID SPECIES
32089 Drosophila melanogaster
427803 Gallus gallus
495437 Xenopus laevis
738821 Pan troglodytes
828239 Arabidopsis thaliana
100058875 Equus caballus
100357790 Oryctolagus cuniculus
8877 Homo sapiens
20698 Mus musculus
618605 Bos taurus
170897 Rattus norvegicus
483329 Canis lupus familiaris
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