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Characteristics
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Ni-charged MagBeads (Cat. No. L00295, 8 ml 25% slurry) are average 40µm in size, superparamagnetic beads pre-charged with nickel ions and ready to use for quick and small-scale purification of His-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Ni-Charged MagBeads have a binding capacity of 5 to 20 mg 6×His-tagged protein per 1 ml settled beads (e.g. 4 ml 25% slurry).The beads are compatible with magnetic separation-based high throughput applications. Proteins can be purified under non-denaturing conditions or in the presence of 8 M urea.
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