IL1R2 antibody

Catalog No. ABIN103286

Product Details anti-IL1R2 Antibody

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1R2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics:

Synonyms: rabbit anti-IL-1 Receptor Type II Antibody, rabbit anti-interleukin-1 receptor beta type II antibody, IL1R Beta antibody, Il1r-2 antibody, IL1R2 antibody, IL1RB antibody, Interleukin 1 receptor beta type II antibody

Background: Interleukin 1 receptor type II binds to IL-1 alpha, IL-1 beta and IL1R1/IL1RA. Anti-IL-1 Receptor Type II antibody is ideal for investigators involved in Immunology and Stem Cell research.

Gene Name: IL1R2

Purification: This is an IgG preparation of whole rabbit serum purified by Protein A chromatography.  The antibody is directed against the extracellular domain of the human IL-1R type II, but precipitates the intact receptor expressed on human cells.   In general, this antibody also detects primate IL-1RII.  The antibody does not recognize human IL-1, IL-1Ra or IL-1R type I.  In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type II (also called soluble IL-1R type II) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.   This antibody can be used for FACS analysis.  Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1R type II.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This IgG fraction antibody of anti-Human IL-1 Receptor Type II (IL-1RII) has been tested for use in ELISA,  radioimmunoassays, flow cytometry and immunoprecipitation.  Reactivity in other immunoassays is unknown.   IL-1RII has been termed p68 or CD121 in some laboratories.  IL-1RII has been shown to bind IL-1, IL-1a and IL-1b.This product has been assayed for the ability to immunoprecipitate antigen.  A dilution range of 1:400 to 1:800 is suggested for this immunoassay.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against soluble (extracellular) IL-1RII using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2000 is suggested for this product.  For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay.  This product has been assayed for the ability to immunoprecipitate antigen.  A dilution range of 1:400 to 1:800 is suggested for this immunoassay.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against soluble (extracellular) IL-1RII using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2000 is suggested for this product.  For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay. 

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for IL1R2

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Alternative Name: IL-1 Receptor Type II (IL1R2 Products)

Synonyms: CD121b antibody, CDw121b antibody, IL-1R-2 antibody, IL-1RT-2 antibody, IL-1RT2 antibody, IL1R2c antibody, IL1RB antibody, Il1r-2 antibody, IL-1R2 antibody, interleukin 1 receptor type 2 antibody, interleukin 1 receptor, type II antibody, interleukin-like antibody, IL1R2 antibody, Il1r2 antibody, KK34 antibody

Gene ID: 7850

UniProt: P27930

Pathways: NF-kappaB Signaling