Interleukin 33 antibody (IL33) (Biotin)

Details for Product anti-IL33 Antibody No. ABIN1043805, Supplier: Log in to see
Antigen
  • IL33
  • C9orf26
  • DVS27
  • IL1F11
  • NF-HEV
  • NFEHEV
  • RP11-575C20.2
  • 9230117N10Rik
  • Il-33
  • Il1f11
  • RGD1311155
  • interleukin 33
  • interleukin 33-like
  • interleukin-33-like
  • IL33
  • Il33
  • LOC100356081
  • LOC100518643
Alternatives
anti-Human Interleukin 33 antibody for Blocking Antibody
Reactivity
Human
289
106
45
8
4
3
2
2
Host
Rabbit
187
133
24
23
4
3
2
1
1
Clonality
Polyclonal
Conjugate
This Interleukin 33 antibody is conjugated to Biotin
35
19
14
14
8
7
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
278
214
89
61
45
24
21
21
16
14
13
11
7
5
5
4
3
3
2
2
2
1
1
Options
Supplier
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Immunogen This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
Immunogen Type: RecombinantProtein
Isotype IgG
Specificity This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics IL-33 (also known as Interleukin-33, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV) is a cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 induces T helper type 2-associated cytokines. IL-33 is a secreted cytokine that is expressed at high levels in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes and is almost undetectable in placenta. The 31 kDa precursor is proteolytically converted to an 18 kDa mature form by CASP1.
Purification purified
Alternative Name IL-33 (IL33 Antibody Abstract)
Background IL-33 (also known as Interleukin-33, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV) is a cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 induces T helper type 2-associated cytokines. IL-33 is a secreted cytokine that is expressed at high levels in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes and is almost undetectable in placenta. The 31 kDa precursor is proteolytically converted to an 18 kDa mature form by CASP1.
Synonyms: Interleukin-33, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV
Gene ID 90865, 15559209
UniProt O95760
Pathways Production of Molecular Mediator of Immune Response
Application Notes This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 18 kDa in size corresponding to mature human IL-32α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Comment

Gene Name: IL33

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Interleukin 33 antibody (IL33)  (Biotin) (ABIN1043805) Western blot using anti-Human IL-33 antibody shows detection of a band ~18 kDa in siz...
Product cited in: Hayakawa, Hayakawa, Matsuyama, Tamemoto, Okazaki, Tominaga: "Mature interleukin-33 is produced by calpain-mediated cleavage in vivo." in: Biochemical and biophysical research communications, Vol. 387, Issue 1, pp. 218-22, 2009 (PubMed).