RFP antibody (Red Fluorescent Protein) (AA 234)

Details for Product anti-RFP Antibody No. ABIN1043926, Supplier: Log in to see
Antigen
Epitope
AA 234
30
6
3
2
2
1
Reactivity
Discosoma
82
23
21
10
7
2
1
1
Host
Chicken
69
38
14
8
1
Clonality
Polyclonal
Conjugate
This RFP antibody is un-conjugated
7
5
5
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
106
70
47
39
32
29
7
5
4
3
2
1
1
1
1
1
1
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Immunogen Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type: Recombinant Protein
Isotype IgG
Specificity This product was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above.  Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.
Characteristics Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS). This product shows optimal performance by western blot.
Sterility Sterile filtered
Alternative Name RFP
Background Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.
Gene ID 55976617
UniProt Q9U6Y8
Research Area Tags/Labels
Application Notes Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
Comment

Gene Name: DsRed

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.
Expiry Date 3 months
Supplier Images
Western Blotting (WB) image for anti-RFP antibody (Red Fluorescent Protein) (AA 234) (ABIN1043926) Western blot of Anti-RFP Antibody. 0.1 µg of RFP was loaded on a 4-20% gel and tranfe...
 image for anti-RFP antibody (Red Fluorescent Protein) (AA 234) (ABIN1043926) anti-Red Fluorescent Protein (RFP) (AA 234) antibody (Image 2)
Product cited in: Haller, Hock, Giampazolias, Oberst, Green, Debnath, Ryan, Vousden, Tait: "Ubiquitination and proteasomal degradation of ATG12 regulates its proapoptotic activity." in: Autophagy, Vol. 10, Issue 12, pp. 2269-78, 2015 (PubMed).

Gross, Baird, Hoffman, Baldridge, Tsien: "The structure of the chromophore within DsRed, a red fluorescent protein from coral." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 22, pp. 11990-5, 2000 (PubMed).