CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C) (APC)

Details for Product anti-PTPRC Antibody No. ABIN1106393, Supplier: Log in to see
Antigen
  • PTPRC
  • si:dkeyp-47c5.1
  • B220
  • CD45
  • CD45R
  • GP180
  • L-CA
  • LCA
  • LY5
  • T200
  • Cd45
  • Ly-5
  • Lyt-4
  • loc
  • Lca
  • RT7
  • cd45
  • protein tyrosine phosphatase, receptor type, C
  • PTPRC
  • ptprc
  • Ptprc
Alternatives
anti-Human CD45 antibody for Biochemical Assay
Reactivity
Human
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713
185
128
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45
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Host
Mouse
1377
737
235
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is conjugated to APC
252
222
167
90
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61
54
48
41
36
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Application
Flow Cytometry (FACS)
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536
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Options
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Clone ML2
Isotype IgG1
Specificity Anti-CD45 (clone ML2) reacts with the CD45 antigen. CD45 clustered antibodies have proven valuable for gate setting of lymphocytes, leukocytes, and monocytes in routine testing using flow cytometry. Combination of CD45 with CD14 antibodies in the analysis of blood (or bone marrow) samples by flow cytometry shows variable expression of these antigens on different cell populations. In peripheral blood cells, a distinction can be made between lymphocytes (CD45+++, CD14 -), monocytes (CD45++, CD14++) and granulocytes (CD45++, CD14+/-). Testing by flow cytometry using a 'lyse-wash' method on whole blood from healthy donors showed the following values expressed in terms of % of the total lymphocyte count: Product code: AM39019AC-N (anti-CD45 APC) n: 10 Mean % positive: 99,72 S.D.: 0,30 % CV: 0,31 ------------------------------------------ Product code: AM39019FC-N (anti-CD45 FITC) n: 10 Mean % positive: 99,43 S.D.: 0,66 % CV: 0,66 ------------------------------------------ Product code: AM39019RP-N (anti-CD45 PE) n: 10 Mean % positive: 99,75 S.D.: 0,20 % CV: 0,21 ------------------------------------------
Purification Affinity chromatography
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background The CD45 Molecule is also known as the Leukocyte Common Antigen (LCA) or T200 antigen, and is comprised of different glycoproteins ranging from 180-240 kD [1,2]. CD45 is a family of transmembrane protein tyrosine phosphatases critically involved in the regulation of lymphocyte activation signals. Expression of CD45 is found on all hemopoietic cells, e.g. granulocytes, monocytes, macrophages and lymphocytes, except mature erythroid cells. In humans, there is heterogeneous expression of CD45 isoforms (RA, RB, RO, RC) on lymphocyte subpopulations. Detection of the different isoforms can distinguish, for example, between naive T cells and memory T cells, which is of interest in patients with immunodeficiency and autoimmune diseases.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 5788
NCBI Accession NP_002829
UniProt P08575
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.
Supplier Images
 image for anti-CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C)  (APC) (ABIN1106393) anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody (APC)
Background publications Neel: "Role of phosphatases in lymphocyte activation." in: Current opinion in immunology, Vol. 9, Issue 3, pp. 405-20, 1997 (PubMed).

Thomas: "The leukocyte common antigen family." in: Annual review of immunology, Vol. 7, pp. 339-69, 1989 (PubMed).

Pingel, Thomas: "Evidence that the leukocyte-common antigen is required for antigen-induced T lymphocyte proliferation." in: Cell, Vol. 58, Issue 6, pp. 1055-65, 1989 (PubMed).

Streuli, Hall, Saga, Schlossman, Saito: "Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens." in: The Journal of experimental medicine, Vol. 166, Issue 5, pp. 1548-66, 1987 (PubMed).