GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN1107349, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
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1
Host
Mouse
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9
2
Clonality (Clone)
Monoclonal ()
Conjugate
This GFP antibody is un-conjugated
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28
14
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1
1
1
1
1
1
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1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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8
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3
2
2
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Options
Supplier
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Supplier Product No.
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Immunogen Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) from the Jellyfish Aequorea victoria.
Clone N-a
Isotype IgG1
Purification Affinity Purified
Alternative Name GFP
Target Type Tag
Background The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. When expressed in cells, fluorescence is produced without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. A number of GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wild type GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. Thus, GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.Synonyms: GFP-Tag, Green fluorescent protein
Gene ID 6100
UniProt P42212
Research Area Tags/Labels
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.2), 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1107349) anti-Green Fluorescent Protein (GFP) antibody
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1107349) anti-Green Fluorescent Protein (GFP) antibody (Image 2)
Background publications Ouyang, Wong, Capani, Rensing, Lee, Liu, Neusch, Martone, Wu, Yamada, Ellisman, Choi: "Transient decrease in F-actin may be necessary for translocation of proteins into dendritic spines." in: The European journal of neuroscience, Vol. 22, Issue 12, pp. 2995-3005, 2005 (PubMed).

Jurado, Haserick, Lee: "Slipping or gripping? Fluorescent speckle microscopy in fish keratocytes reveals two different mechanisms for generating a retrograde flow of actin." in: Molecular biology of the cell, Vol. 16, Issue 2, pp. 507-18, 2005 (PubMed).

Wang, Bhattacharyya, Chelsea, Escobar, Banerjee: "A novel nuclear protein, MGC5306 interacts with DNA polymerase beta and has a potential role in cellular phenotype." in: Cancer research, Vol. 64, Issue 21, pp. 7673-7, 2004 (PubMed).

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