GJB2 antibody (Middle Region)

Catalog No. ABIN1107369

Product Details anti-GJB2 Antibody

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody detects GJB2 / Cx26 (mid region). No cross reactivity with other proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Immunogen affinity purified

Immunogen: Peptide mapping at the middle region of rat Connexin 26 .Remarks: Sequence is different from the relative sequence of human by three amino acids.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: 1.2 % sodium acetate or neutral PBS. If 0.5 mL of PBS is used, the antibody concentration will be 100 μg/mL.

Buffer: 50 % glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for GJB2

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Alternative Name: GJB2 / Cx26 (GJB2 Products)

Synonyms: ppk antibody, cx26 antibody, dfna3 antibody, dfnb1 antibody, nsrd1 antibody, dfna3a antibody, dfnb1a antibody, MGC53062 antibody, connexin-26 antibody, GJB2 antibody, AI325222 antibody, Cnx26 antibody, Cx26 antibody, Gjb-2 antibody, CXN-26 antibody, CX26 antibody, DFNA3 antibody, DFNA3A antibody, DFNB1 antibody, DFNB1A antibody, HID antibody, KID antibody, NSRD1 antibody, PPK antibody, gap junction protein beta 2 L homeolog antibody, gap junction protein beta 2 antibody, gap junction protein, beta 2 antibody, gjb2.L antibody, GJB2 antibody, Gjb2 antibody

Background: Connexin26(CX26), also known as GAP junction protein, beta2, GJB2. Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins,called connexins, purified from fractions of enriched gap junctions from different tissues differ. The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence. The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD. CX26 shares 92.5 % identity with rat Cx26. connexin 26 (GJB2) is assigned to human chromosome 13q11-q12. Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. Connexin 26 gene (GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation.Synonyms: Connexin-26, Gap junction beta-2 protein

Gene ID: 2706

NCBI Accession: NP_003995

UniProt: P29033

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization