HSPD1 antibody

Catalog No. ABIN1107605

Product Details anti-HSPD1 Antibody

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Human, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody reacts to HSP60.

Purification: Affinity chromatography

Immunogen: Recombinant human heat shock protein 60 (HSP60)

Clone: SJ-60

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.2 % sodium acetate or neutral PBS

Concentration: 0,1 mg/mL

Buffer: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSP60 (HSPD1 Products)

Synonyms: CPN60 antibody, GROEL antibody, HLD4 antibody, HSP-60 antibody, HSP60 antibody, HSP65 antibody, HuCHA60 antibody, SPG13 antibody, chaperonin antibody, cpn60 antibody, groel antibody, hld4 antibody, hsp60 antibody, hsp65 antibody, spg13 antibody, 60kDa antibody, Hsp60 antibody, 12 antibody, BP5 antibody, CG12101 antibody, Cpn60 antibody, Dmel\\CG12101 antibody, Dmhsp60 antibody, G62 antibody, HSP60A antibody, Hsp60A antibody, IEF16 antibody, Mmp-P1 antibody, SSP 7506 antibody, d-hsp60 antibody, hsp60A antibody, l(1)10Ac antibody, l(1)BP5 antibody, l(1)G8 antibody, l(1)HM21 antibody, l(1)L12 antibody, l(1)dp025 antibody, cb863 antibody, fa04a05 antibody, fb22d10 antibody, fi27b05 antibody, id:ibd2197 antibody, sb:cb144 antibody, wu:fa04a05 antibody, wu:fb22d10 antibody, wu:fi04a12 antibody, wu:fi27b05 antibody, MIF4 antibody, MNA2 antibody, mopA antibody, groL antibody, crpA antibody, Hspd1-30p antibody, heat shock protein family D (Hsp60) member 1 antibody, heat shock protein family D (Hsp60) member 1 S homeolog antibody, 60 kDa heat shock protein, mitochondrial antibody, heat shock protein 1 (chaperonin) antibody, Heat shock protein 60A antibody, heat shock 60 protein 1 antibody, chaperone ATPase HSP60 antibody, molecular chaperone GroEL antibody, thermosome subunit antibody, chaperonin GroEL antibody, mitochondrial chaperonin antibody, heat shock protein family D member 1 antibody, HSPD1 antibody, hspd1.S antibody, hspd1 antibody, LOC100414401 antibody, Hspd1 antibody, Hsp60A antibody, HSP60 antibody, groEL antibody, MMP_RS07785 antibody, groEl antibody, LOC100136430 antibody

Background: Heat shock 60KD protein (HSP60) is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNFsecretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.Synonyms: 60 kDa chaperonin, 60 kDa heat shock protein mitochondrial, CPN60, Chaperonin 60, GROEL, GroEL Homolog, HSP-60, HSPD1, Heat shock protein 60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein

Gene ID: 3329

NCBI Accession: NP_002147

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity