TSFM antibody (N-Term)

Catalog No. ABIN1109361

The Rabbit Polyclonal anti-TSFM antibody (ABIN1109361) specifically detects TSFM in WB and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio) and Xenopus laevis samples.

Quick Overview for TSFM antibody (N-Term) (ABIN1109361)

Target: TSFM (Ts Translation Elongation Factor, Mitochondrial (TSFM))

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSFM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TSFM Antibody

Binding Specificity: N-Term

Sequence: KQAEIWLHKE AQKEGWSKAA KLQGRKTKEG LIGLLQEGNT TVLVEVNCET

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, African clawed frog, Bovine, ZebrafishSpecies reactivity (tested):Human

Purification: Purified using peptide immunoaffinity column

Immunogen: The immunogen for anti-TSFM antibody: synthetic peptide directed towards the N terminal of human TSFM.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Add 50 μL of distilled water to a final concentration of 1 mg/mL.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C or at -20 °C long term. After reconstitution store the antibody undiluted at 2-8 °C for up to one month or in aliquots at -20 °C long term.

Target Details for TSFM

Target: TSFM (Ts Translation Elongation Factor, Mitochondrial (TSFM))

Alternative Name: TSFM

Background: The TSFM gene encodes a protein that is expressed in all tissues, with the highest levels of expression in skeletal muscle, liver, and kidney. It associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. Defects in TSFM are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3). Defects in the mitochondrial oxidative phosphorylation system result in devastating, mainly multisystem, diseases. COXPD3 symptoms include severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Patients show a severe defect in mitochondrial translation leading to a failure to assemble adequate amounts of three of the oxidative phosphorylation complexesSynonyms: EF-Ts, EF-TsMt, Elongation factor Ts mitochondrial

Gene ID: 10102

NCBI Accession: NP_005717