TSFM antibody (Middle Region)

Catalog No. ABIN2777309

The Rabbit Polyclonal anti-TSFM antibody (ABIN2777309) specifically detects TSFM in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig and Rabbit samples.

Quick Overview for TSFM antibody (Middle Region) (ABIN2777309)

Target: TSFM (Ts Translation Elongation Factor, Mitochondrial (TSFM))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSFM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TSFM Antibody

Binding Specificity: Middle Region

Purpose: TSFM Antibody - middle region

Sequence: GTMMHCQTLK DQPSAYSKGF LNSSELSGLP AGPDREGSLK DQLALAIGKL

Predicted Reactivity: Cow: 86%, Guinea Pig: 86%, Human: 100%, Mouse: 86%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against TSFM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TSFM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8114847

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TSFM

Target: TSFM (Ts Translation Elongation Factor, Mitochondrial (TSFM))

Alternative Name: TSFM

Background:

Background Information: The TSFM protein is a mitochondrial translation elongation factor. Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes.Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes. The TSFM gene encodes a mitochondrial translation elongation factor (Smeitink et al., 2006 [PubMed 17033963]).[supplied by OMIM].

Gene Name: Ts translation elongation factor, mitochondrial

Alternative Symbols: EFTS, EFTSMT

Protein Name: Elongation factor Ts, mitochondrial

Molecular Weight: 38kDa

Gene ID: 10102

NCBI Accession: NP_005717

UniProt: P43897