ELANE antibody

Catalog No. ABIN111823

Product Details anti-ELANE Antibody

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ELANE antibody is un-conjugated

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody is specific for Neutrophil Elastase.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Elastase from Human Neutrophils.

Clone: 39A

Isotype: IgG1

Application Details

Application Notes: ELISA. Flow Cytometry. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ELANE

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Alternative Name: Neutrophil Elastase (ELANE Products)

Synonyms: ELA2 antibody, GE antibody, HLE antibody, HNE antibody, NE antibody, PMN-E antibody, SCN1 antibody, Ela2 antibody, F430011M15Rik antibody, ela2 antibody, ELANE antibody, elastase, neutrophil expressed antibody, proteinase 3 antibody, ELANE antibody, Elane antibody, prtn3 antibody

Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5 kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.Synonyms: Bone marrow serine protease, ELA2, ELANE, Elastase-2, HLE, Human leukocyte elastase, Medullasin, Neutrophil elastase, PMN elastase

Gene ID: 1991

NCBI Accession: NP_001963

UniProt: P08246